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Art. -Nr.: HY-101866

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KKL-35

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KKL-35 is a trans-translation tagging reaction inhibitor with an IC₅₀ of 0.9 μM.

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Reinheit: 99.46%
CAS. Nr.: 865285-29-6
Formel: C₁₅H₉ClFN₃O₂
Molecular Weight:317.70

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

KKL-35 Chemical Structure

CAS. Nr.: 865285-29-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

KKL-35 is a trans-translation tagging reaction inhibitor with an IC₅₀ of 0.9 μM.

IC₅₀ & Target

IC50: 0.9 μM (trans-translation tagging reaction)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	14 μM Compound: KKL-35	Cytotoxicity against human Caco2 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human Caco2 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
HCT-116	IC₅₀	17 μM Compound: KKL-35	Cytotoxicity against human HCT116 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human HCT116 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
HEK293	IC₅₀	8.9 μM Compound: 42	Inhibition of human AC1 expressed in HEK293 cells assessed as decrease in A23187-stimulated cAMP accumulation preincubated for 30 mins followed by A23187 stimulation and measured after 1 hr by fluorescence assay Inhibition of human AC1 expressed in HEK293 cells assessed as decrease in A23187-stimulated cAMP accumulation preincubated for 30 mins followed by A23187 stimulation and measured after 1 hr by fluorescence assay	[PMID: 30472604]
Huh-7	IC₅₀	20 μM Compound: KKL-35	Cytotoxicity against human HuH7 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human HuH7 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
MCF7	IC₅₀	22 μM Compound: KKL-35	Cytotoxicity against human MCF7 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human MCF7 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
MDA-MB-231	IC₅₀	25 μM Compound: KKL-35	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
NCI-H727	IC₅₀	18 μM Compound: KKL-35	Cytotoxicity against human NCI-H727 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human NCI-H727 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]
PC-3	IC₅₀	21 μM Compound: KKL-35	Cytotoxicity against human PC3 cells assessed as decrease in cell viability in MTT assay Cytotoxicity against human PC3 cells assessed as decrease in cell viability in MTT assay	[PMID: 31540826]

In Vitro

KKL-35 exhibits broad-spectrum antibiotic activity. KKL-35 prevents growth of B. anthracis and M. smegmatis with minimum inhibitory concentration (MIC) values of less than 6 μM. KKL-35 inhibit trans-translation at some step before proteolysis of tagged proteins. KKL-35 inhibits tagging of DHFR-ns. A large amount of untagged DHFR is produced in reactions with the highest concentrations of KKL-35, indicating that KKL-35 does not inhibit translation. KKL-35 prevents growth of WT S. flexneri with a MIC of 6 μM, and addition of KKL-35 to a growing culture of S. flexneri stops growth. In an S. flexneri strain expressing ArfA and deleted for ssrA, addition of KKL-35 has little effect on viability or growth rate. KKL-35 inhibits the growth of E. coli tolC, which is deficient in small molecule efflux, with an MIC of 0.3 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Evidence suggest that the in vivo effects of KKL-35 are caused by inhibition of the release of nonstop translation complexes by trans-translation. KKL-35 inhibits trans-translation and prevents growth of S. flexneri strains that require trans-translation. The correlation between inhibition of trans-translation and growth is supported by genetic and pharmacological experiments showing that alternative mechanisms to release nonstop translation complexes relieve the growth suppression of KKL-35^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 865285-29-6
Appearance Solid
Molecular Weight 317.70
Formel C₁₅H₉ClFN₃O₂
Color White to light yellow
SMILES

O=C(NC1=NN=C(C2=CC=C(F)C=C2)O1)C3=CC=C(Cl)C=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 6.25 mg/mL (19.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1476 mL

15.7381 mL

31.4762 mL

5 mM

0.6295 mL

3.1476 mL

6.2952 mL

10 mM

0.3148 mL

1.5738 mL

3.1476 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ramadoss NS, et al. Small molecule inhibitors of trans-translation have broad-spectrum antibiotic activity. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10282-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1476 mL	15.7381 mL	31.4762 mL	78.6906 mL
	5 mM	0.6295 mL	3.1476 mL	6.2952 mL	15.7381 mL
	10 mM	0.3148 mL	1.5738 mL	3.1476 mL	7.8691 mL
	15 mM	0.2098 mL	1.0492 mL	2.0984 mL	5.2460 mL

Keywords

MOQ

Minimum order quantity

100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

Produktname:
Art. -Nr.:
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KKL-35

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

KKL-35 Related Classifications

HY-101866 Related Classifications