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  4. L-Homocysteine thiolactone hydrochloride

L-Homocysteine thiolactone (hydrochloride)  (Synonyms: L-ホモシステインチオラクトン 塩酸塩)

製品番号: HY-101404A 純度: 99.99%
COA 取扱説明書 Technical Support

L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice.

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L-Homocysteine thiolactone hydrochloride

L-Homocysteine thiolactone (hydrochloride) 構造式

CAS 番号 : 31828-68-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
10 mg $30 在庫あり
25 mg $55 在庫あり
50 mg $78 在庫あり
100 mg $100 在庫あり
250 mg $165 在庫あり
1 g $495 在庫あり
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L-Homocysteine thiolactone (hydrochloride) 関連抗体

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製品説明

L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice[1][2][3].

体外実験

L-Homocysteine thiolactone hydrochloride (20 μM; for 24 h) shows significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6[2].
L-Homocysteine thiolactone hydrochloride (100 μM; for 24 h) shows significantly higher ratios of nuclear/cytoplasmic NF-κB, indicating that Homocysteine (Hcy)-induced NF-κB activation and nuclear translocation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Homocysteine thiolactone (hydrochloride) 関連抗体

Western Blot Analysis[2]

Cell Line: U937 cells
Concentration: 100 μM
Incubation Time: 24 h
Result: Showed significantly higher ratios of nuclear/cytoplasmic NF-κB.

RT-PCR[2]

Cell Line: U937 cells
Concentration: 20 μM
Incubation Time: 24 h
Result: Showed significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6.
体内実験

L-Homocysteine thiolactone hydrochloride can be used to create models of hyperhomocysteinemia[2].

Induction of Hyperhomocysteinemia (HHcy)[2][3]
Background
Homocysteine (Hcy) is an amino acid that requires vitamins B12 and folic acid for its metabolism. Vitamins B12 and folic acid deficiencies lead to hyperhomocysteinemia (HHcy, elevated Hcy), which is linked to the development of diabetic retinopathy (DR), age-related macular degeneration (AMD), and Alzheimer’s disease (AD).
Specific Modeling Methods
Male C57Mice: cbs+/-; 3-5 weeks • C57BL6/J; 6-8 weeks of age
Administration: 1 μL • intravitreally • 200 μM BL/6 mice (5 weeks old)
Note
(1) To avoid uncontrolled intraocular pressure increase, the volume of intravitreal injections is limited to 1 μL.
(2) L-Homocysteine thiolactone hydrochloride is dissolved in water and a working solution of 10x is prepared by diluting 1 μL of stock solution (200 mM) to 100 μL with PBS. Then by injecting 1 μL of this working solution, 200 μM vitreal concentration of Hcy-thiolactone is obtained.
Modeling Indicators
Molecular changes: Caused microglia activation in the retina. Increased indicators: pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6
Correlated Product(s): L-Homocysteine (HY-W010347)
Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: cbs+/- mice and normal wild type mice (C57BL6/J), 6-8 weeks of age[2]
Dosage: 1 μL; 200 μM
Administration: Intravitreally
Result: Normal retinal flat-mount preparations from cbs+/+ mice showed quiescent ramified microglia, while cbs-/- and cbs+/- retinas demonstrated rod-like microglia devoid of branching processes, indicating microglia activation in HHcy mice.
分子量

153.63

分子式

C4H8ClNOS
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCS1)C1=O.Cl
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

H2O : ≥ 200 mg/mL (1301.83 mM)

DMSO : 116.67 mg/mL (759.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5091 mL 32.5457 mL 65.0915 mL
5 mM 1.3018 mL 6.5091 mL 13.0183 mL
10 mM 0.6509 mL 3.2546 mL 6.5091 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (650.91 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.99%

COA (249 KB) HNMR (238 KB) RP-HPLC (232 KB) MS (69 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.5091 mL 32.5457 mL 65.0915 mL 162.7286 mL
5 mM 1.3018 mL 6.5091 mL 13.0183 mL 32.5457 mL
10 mM 0.6509 mL 3.2546 mL 6.5091 mL 16.2729 mL
15 mM 0.4339 mL 2.1697 mL 4.3394 mL 10.8486 mL
20 mM 0.3255 mL 1.6273 mL 3.2546 mL 8.1364 mL
25 mM 0.2604 mL 1.3018 mL 2.6037 mL 6.5091 mL
30 mM 0.2170 mL 1.0849 mL 2.1697 mL 5.4243 mL
40 mM 0.1627 mL 0.8136 mL 1.6273 mL 4.0682 mL
50 mM 0.1302 mL 0.6509 mL 1.3018 mL 3.2546 mL
60 mM 0.1085 mL 0.5424 mL 1.0849 mL 2.7121 mL
80 mM 0.0814 mL 0.4068 mL 0.8136 mL 2.0341 mL
100 mM 0.0651 mL 0.3255 mL 0.6509 mL 1.6273 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Homocysteine thiolactone hydrochloride Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Homocysteine thiolactone31828-68-9Amino Acid DerivativesHyperhomocysteinemiaHHcyInhibitorinhibitorinhibit

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製品名:
L-Homocysteine thiolactone hydrochloride
製品番号:
HY-101404A
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