L-Homocysteine
Based on 7 publication(s) in Google Scholar
L-Homocysteine, an amino acid, is a homocysteine that has L configuration. Homocysteine is an essential intermediate in normal mammalian metabolism of methionine. L-Homocysteine induces upregulation of Cathepsin V that mediates vascular endothelial inflammation in hyperhomocysteinaemia.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 6027-13-0
- Formula: C4H9NO2S
- Molecular Weight:135.19
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) L-Homocysteine
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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RT-PCR
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WB
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ELISA
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WB
All Endogenous Metabolite Isoforms
MoreAll Cathepsin Isoforms
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Biological Activity
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Human Endogenous Metabolite |
cathepsin L |
L-Homocysteine (0.5, 1, 2 mM; 24 h) increases the mRNA and protein levels of Cathepsin V in a dose‐dependent manner[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HUVECs
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Concentration:0.5, 1, 2 mM
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Incubation Time:24 h
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Result:Increased the mRNA and protein levels of Cathepsin V in a dose‐dependent manner.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
L-Homocysteine can be used in animal modeling to construct a model of vascular endothelial inflammation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6027-13-0
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Appearance Solid
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Molecular Weight 135.19
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Formula C4H9NO2S
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Color White to off-white
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SMILES
N[C@@H](CCS)C(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (7)
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Journal Impact Factor
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Most Recent
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J Chromatogr A
Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS. [Abstract]2025 May 10:1755:466036. PMID: 40398135 -
J Chromatogr A
Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS. [Abstract]2024 Apr 12:1720:464776. PMID: 38432109 -
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Exp Cell Res
Ciliary neurotrophic factor promotes the development of homocysteine-induced vascular endothelial injury through inflammation mediated by the JAK2/STAT3 signaling pathway. [Abstract]2024 Jun 5:114103. PMID: 38848951
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
CCK-8 assay evaluated the vitality of HUVECs under various concentrations of L-Homocysteine (Hcy; 2.5, 5 or 7.5 mM; 24; 48, 72 h).
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
qPCR evaluated the expression of CNTF in HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
Western blotting evaluated the expression of CNTF in HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
ELISA was used to measure the levels of CNTF in the culture medium of HUVECs treated with L-Homocysteine (Hcy; 7.5 mM; 24 h).
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
Western blotting was employed to analyze the levels of p-JAK2, JAK2, p-STAT3, STAT3, and IL-6 in HUVECs exposed to varying concentrations of L-Homocysteine (Hcy; 7.5 mM; 24 h).
L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103. [Abstract]
Flow cytometry detected the apoptosis of HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).
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J Pharm Biomed Anal
A rapid LC-MS/MS method for the simultaneous quantification of 10 key folate cycle intermediates in human plasma. [Abstract]2025 Oct 3:268:117178. PMID: 41061512 -
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Solvent & Solubility
H2O : 25 mg/mL (184.92 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (369.85 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.3970 mL | 36.9850 mL | 73.9700 mL | 184.9249 mL |
| 5 mM | 1.4794 mL | 7.3970 mL | 14.7940 mL | 36.9850 mL | |
| 10 mM | 0.7397 mL | 3.6985 mL | 7.3970 mL | 18.4925 mL | |
| 15 mM | 0.4931 mL | 2.4657 mL | 4.9313 mL | 12.3283 mL | |
| 20 mM | 0.3698 mL | 1.8492 mL | 3.6985 mL | 9.2462 mL | |
| 25 mM | 0.2959 mL | 1.4794 mL | 2.9588 mL | 7.3970 mL | |
| 30 mM | 0.2466 mL | 1.2328 mL | 2.4657 mL | 6.1642 mL | |
| 40 mM | 0.1849 mL | 0.9246 mL | 1.8492 mL | 4.6231 mL | |
| 50 mM | 0.1479 mL | 0.7397 mL | 1.4794 mL | 3.6985 mL | |
| 60 mM | 0.1233 mL | 0.6164 mL | 1.2328 mL | 3.0821 mL | |
| 80 mM | 0.0925 mL | 0.4623 mL | 0.9246 mL | 2.3116 mL | |
| 100 mM | 0.0740 mL | 0.3698 mL | 0.7397 mL | 1.8492 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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