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LCL521 dihydrochloride (Synonyms: 1,3DMG-B13 dihydrochloride)

Cat. No.: HY-103593A
Handling Instructions

LCL521 dihydrochloride is an acid ceramidase (ACDase) inhibitor. LCL521 also inhibits the lysosomal acid sphingomyelinase (ASMase).

For research use only. We do not sell to patients.

LCL521 dihydrochloride Chemical Structure

LCL521 dihydrochloride Chemical Structure

CAS No. : 1226759-47-2

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

LCL521 dihydrochloride is an acid ceramidase (ACDase) inhibitor. LCL521 also inhibits the lysosomal acid sphingomyelinase (ASMase).

IC50 & Target

ACDase, ASMase[1]

In Vitro

Acid ceramidase (ACDase) is being recognized as a therapeutic target for cancer. B13 represents a moderate inhibitor of ACDase. LCL521 applied at a lower concentration, 1 µM, shows an effect similar to 10µM treatment, suggesting a high efficiency on this enzyme inhibition. LCL521 causes a time-dependent decrease of the α-ACDase form. 1 µM LCL521 acts as a potent inhibitor of cellular ACDase activity, whereas 10 µM LCL521 has an additional, decreased affect on the α-form of this enzyme. Moreover, LCL521 acts as inhibitor of the lysosomal ACDase and ASMase[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5022 mL 7.5110 mL 15.0220 mL
5 mM 0.3004 mL 1.5022 mL 3.0044 mL
10 mM 0.1502 mL 0.7511 mL 1.5022 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

HeLa cells overexpressing ASAH2, ACER2, or empty vector are grown to a 60% confluence before they are treated with Tetracycline (5 ng/mL) overnight. Next day, the cells are then treated with B13 (10 µM), LCL521 (1 or 10 µM), or Ethanol (the vehicle control for both B13 and LCL521) for 1 h before they are harvested[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

665.69

Formula

C₃₁H₅₄Cl₂N₄O₇

CAS No.

1226759-47-2

SMILES

O=C(CN(C)C)O[[email protected]](C1=CC=C([N+]([O-])=O)C=C1)[[email protected]@H](COC(CN(C)C)=O)NC(CCCCCCCCCCCCC)=O.[H]Cl.[H]Cl

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
LCL521 dihydrochloride
Cat. No.:
HY-103593A
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LCL521 dihydrochloride

Cat. No.: HY-103593A