Luciferase, firefly 

Luciferase, firefly is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. Luciferase, firefly catalytic substrate is D-luciferin (HY-12591B)^[1].

Luciferase, firefly monitors changes in ATP concentration in different regions within HL-1 cells and outside the cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Luciferase, firefly (labels HEK293 cells stably expressing pmeLUC; i.p. or intramass injection) can measure the ATP concentration in the tumor microenvironment in real-time in the mouse tumor model inoculated with HEK293 cells stably expressing pmeLUC^[1].
Luciferase, firefly can clearly monitor the growth of cancer cells in a mouse model in which RENCA^LUC cells are inoculated subcutaneously^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

61970-00-1

Solid

White to off-white

[Luciferase, firefly]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Morciano G, et al. Use of luciferase probes to measure ATP in living cells and animals. Nat Protoc. 2017 Aug;12(8):1542-1562.  [Content Brief]

  [2]. Nogawa M, et al. Monitoring luciferase-labeled cancer cell growth and metastasis in different in vivo models. Cancer Lett. 2005 Jan 20;217(2):243-53.  [Content Brief]