Lysozyme from chicken egg white

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Lysozyme from chicken egg white is a bactericidal enzyme, and it lyses gram-positive bacteria. Lysozyme from chicken egg white can also be used for the research of HIV infection and pulmonary emphysema.

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CAS. Nr. : 12650-88-3

Größe	Verfügbarkeit	Menge
500 mg	Auf Lager	Estimated Time of Arrival: December 31
1 g	Auf Lager	Estimated Time of Arrival: December 31
5 g	Auf Lager	Estimated Time of Arrival: December 31
10 g	Auf Lager	Estimated Time of Arrival: December 31
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Based on 10 publication(s) in Google Scholar

Other Forms of Lysozyme from chicken egg white:

Lysozyme chloride In-stock

10 Publications Citing Use of MCE Lysozyme from chicken egg white

Bio/Physico-chemical Assay

: Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.; Amount of RNA in ng per 10⁶ cells from 5 mL N. europaea cultures extracted with NucleoSpin RNA XS kit preceded by enzymatic digestion with lysozyme (1-3 mg/mL) solution. The effect on RNA yield and quality of different lysozyme concentrations, different volumes of RA1 buffer after lysozyme digestion and different lysozyme incubation times are shown.

: Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.; Examples of electropherograms generated from RNA samples with the Agilent BioAnalyzer 2100 represents an RNA sample extracted with the NS XS kit after pretreatment with a 1 mg/mL lysozyme solution and 15 min of incubation at 37°C with a RIN of 10.0.

: Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.; The protocol on N. winogradskyi cultures using higher lysozyme concentrations, with different incubation times with 1 mg/mL lysozyme solution.

: Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.; The protocol on N. winogradskyi cultures using higher lysozyme concentrations, with the addition of Proteinase K (ProtK) to a 15 mg/mL lysozyme solution and/or vortexing (indicated by V).

: Lysozyme from chicken egg white purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105311.; The protocol on N. winogradskyi cultures using various lysozyme concentrations during shaking incubation at 1,400 rpm for 15 min.

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Beschreibung	Lysozyme from chicken egg white is a bactericidal enzyme, and it lyses gram-positive bacteria. Lysozyme from chicken egg white can also be used for the research of HIV infection and pulmonary emphysema^[1]^[2]^[3].
IC₅₀ & Target	Bacteria^[1]
In Vitro	Lysozyme is an ubiquitous enzyme. The hen egg is the most abundant source of Lysozyme, which constitutes approximately 3.4% of the albumen proteins. Lysozyme is a natural antimicrobial that hydrolyzes the β(1-4) glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine found in the peptidoglycan layer of the bacterial cell wall and causing cell lysis. The bactericidal effect of Lysozyme is primarily limited to gram-positive bacteria, including pathogens such as Listeria monocytogenes and certain Clostridium species as well as some spoilage organisms, including thermophilic spore-forming bacteria and certain yeasts. The gram-negative bacteria are more resistant to Lysozyme action because of their complex cell wall structure^[1]. Lysozyme (1 mg/mL) impairs the ability of hyaluronan (HA) to prevent elastase injury to elastic fibers^[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Lysozyme from chicken egg white Related Antibodies
In Vivo	Syrian hamsters exposes to aerosolized Lysozyme (20 mg in 20 ml of water; 50 min) prior to elastase administration showes significantly increased airspace enlargement^[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
CAS. Nr.	12650-88-3
Appearance	Solid
Color	White to off-white
SMILES	[Lysozyme(chicken egg white)]
Structure Classification	Others
Initial Source	Animals chicken egg white
Versand	Room temperature in continental US; may vary elsewhere.
Speicherung	Please store the product under the recommended conditions in the Certificate of Analysis.
Lösungsmittel & Löslichkeit	In Vitro: H₂O : 10 mg/mL (Need ultrasonic and warming) Molaritätsrechner Verdünnungsrechner Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: [email protected] Technical Support: [email protected]
Reinheit & Dokumentation	Select Batch: Data Sheet (271 KB) SDS (419 KB) COA (242 KB) Handling Instructions (2659 KB)
Verweise	[1]. Banerjee P, et al. Influence of carbon dioxide on the activity of chicken egg white lysozyme. Poult Sci. 2011 Apr;90(4):889-95. [Content Brief] [2]. Jing T, et al. Magnetic molecularly imprinted nanoparticles for recognition of lysozyme. Biosens Bioelectron. 2010 Oct 15;26(2):301-6. [Content Brief] [3]. Cantor JO, et al. The effect of lysozyme on elastase-mediated injury. Exp Biol Med (Maywood). 2002 Feb;227(2):108-13. [Content Brief]

Kinase-Assay ^[1]	For measurement of lytic activity in egg white at each pH, temperature, and CO₂ condition, eggs are randomly selected from a flat of eggs (2 dozen eggs) obtained from a local grocery store. To determine the amount of egg white to be added to obtain a 0.001% lysozyme concentration, it is documented that chicken egg white contains approximately 3.4% lysozyme. For determining egg white activity, 0.030 g of albumen was added to 100 mL of the buffered solutions. This equated to a concentration of approximately 0.001% lysozyme. In addition, the egg white contains other antimicrobial proteins that are naturally present, as mentioned in the Introduction section^[1]. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
Verweise	[1]. Banerjee P, et al. Influence of carbon dioxide on the activity of chicken egg white lysozyme. Poult Sci. 2011 Apr;90(4):889-95. [Content Brief] [2]. Jing T, et al. Magnetic molecularly imprinted nanoparticles for recognition of lysozyme. Biosens Bioelectron. 2010 Oct 15;26(2):301-6. [Content Brief] [3]. Cantor JO, et al. The effect of lysozyme on elastase-mediated injury. Exp Biol Med (Maywood). 2002 Feb;227(2):108-13. [Content Brief]

Kinase-Assay
^[1]

For measurement of lytic activity in egg white at each pH, temperature, and CO₂ condition, eggs are randomly selected from a flat of eggs (2 dozen eggs) obtained from a local grocery store. To determine the amount of egg white to be added to obtain a 0.001% lysozyme concentration, it is documented that chicken egg white contains approximately 3.4% lysozyme. For determining egg white activity, 0.030 g of albumen was added to 100 mL of the buffered solutions. This equated to a concentration of approximately 0.001% lysozyme. In addition, the egg white contains other antimicrobial proteins that are naturally present, as mentioned in the Introduction section^[1].

MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.

Verweise

[1]. Banerjee P, et al. Influence of carbon dioxide on the activity of chicken egg white lysozyme. Poult Sci. 2011 Apr;90(4):889-95. [Content Brief]

[2]. Jing T, et al. Magnetic molecularly imprinted nanoparticles for recognition of lysozyme. Biosens Bioelectron. 2010 Oct 15;26(2):301-6. [Content Brief]

[3]. Cantor JO, et al. The effect of lysozyme on elastase-mediated injury. Exp Biol Med (Maywood). 2002 Feb;227(2):108-13. [Content Brief]