1. Induced Disease Models Products GPCR/G Protein Neuronal Signaling Apoptosis
  2. Nervous System Disease Models Dopamine Receptor Apoptosis
  3. Parkinson's Disease Models
  4. MPTP hydrochloride

Chlorhydrate de MPTP est une neurotoxine de dopamine pénétrante dans le cerveau, induisant la maladie de Parkinson. Chlorhydrate de MPTP, précurseur du MPP+, induit l'apoptose.

MPTP-Hydrochlorid ist ein hirneindringendes dopamine-Neurotoxin, das die Parkinson-Krankheit auslöst. MPTP-Hydrochlorid, ein Vorläufer von MPP+, induziert apoptosis.

MPTP hydrochloride is a brain penetrant dopamine neurotoxin. MPTP hydrochloride can be used to induces Parkinson’s Disease model. MPTP hydrochloride, a precusor of MPP+, induces apoptosis. MPTP hydrochloride has been verified by MCE with professional biological experiments.

For research use only. We do not sell to patients.

MPTP hydrochloride Chemical Structure

MPTP hydrochloride Chemical Structure

CAS No. : 23007-85-4

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10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 66 In-stock
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10 mg USD 60 In-stock
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Customer Review

Based on 35 publication(s) in Google Scholar

Top Publications Citing Use of Products

31 Publications Citing Use of MCE MPTP hydrochloride

WB
IHC
IF

    MPTP hydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2019 Jul 15;1715:203-212.  [Abstract]

    Immunofluorescence for TH. The intranigral Apelin-13 injection significantly inhibits MPTP-induced the neurodegeneration of dopaminergic neurons in the SNpc.

    MPTP hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmacol Biochem Behav. 2019 Feb;177:1-11.  [Abstract]

    Effect of treatment with Rapamycin, trehalose, or their combination on tyrosine hydroxylase (TH) expression in the striatum in MPTP-induced mouse model of Parkinson’s disease.

    MPTP hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmacol Biochem Behav. 2019 Feb;177:1-11.  [Abstract]

    Effect of treatment with Rapamycin, trehalose, or their combination on autophagy activity measured by quantified immunoreactivity of LC3-II in the s. nigra. MPTP is administered at the dose of 20 mg/kg (i.p., daily) for 4 days to induce PD-like pathology.

    MPTP hydrochloride purchased from MedChemExpress. Usage Cited in: Brain Res. 2016 Jul 1;1642:546-552.  [Abstract]

    RNA 5hmC decreases in a MPTP-induced Parkinson's disease mouse model. MPTP (i.p. 60 mg/kg) is injected to induce Parkinson's disease model in mice. At 24 h after last MPTP injection, open field test is performed. After behavioral tests, the hippocampus (Hipo), the substantia nigra (SN), the striatum (Str), and the cortex (Ctx) are collected and total RNA is extracted. Total 100 ng RNA is used for dot blot analysis to detect 5hmC abundance in RNA samples from different brain regions. Methylene bl
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MPTP hydrochloride is a brain penetrant dopamine neurotoxin. MPTP hydrochloride can be used to induces Parkinson’s Disease model. MPTP hydrochloride, a precusor of MPP+, induces apoptosis[1][2][3]. MPTP hydrochloride has been verified by MCE with professional biological experiments.

    In Vitro

    Pretreatment with 50 mM 4-phenylpyridine, reduces IC50 (concentration for 50% inhibition of twitch amplitude) values of MPTP from 53 to 18 mM and d-tubocurarine from 0.7 to 0.3 mM, respectively, in mouse phrenic nerve-diaphragm[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Although MPTP can be administered by a variety of different routes, including oral gavage and stereotaxic injection into the brain, the most common, and reproducible, results are obtained by systemic subcutaneous (s.c.) or intraperitoneal (i.p.) injection.
    The commonly used protocols in the references are acute model (14-20 mg/kg, i.p., given every 2 hours within a day, a total of 4 times) and subacute model (30 mg/kg, i.p., once daily for 5 days).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    209.72

    Formula

    C12H16ClN

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CN1CC=C(C2=CC=CC=C2)CC1.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (476.83 mM)

    DMSO : 12 mg/mL (57.22 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7683 mL 23.8413 mL 47.6826 mL
    5 mM 0.9537 mL 4.7683 mL 9.5365 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.67 mg/mL (7.96 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.67 mg/mL (7.96 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: ≥ 100 mg/mL (476.83 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.54%

    References
    Animal Administration
    [3]

    For the preparation of the LPS rat model and the MPTP mouse model, the treatments of the animals are performed. Briefly, adult rats receive unilateral injections of LPS (0.5 μL of 10 μg/μL diluted in 0.9% saline) into the medial forebrain bundle (MFB) at the following coordinates, AP-4.2 mm, L 1.5 mm, and V 7.8 mm, and into the contralateral side with the same volume of 0.9% saline. Adult mice are administered intraperitoneal injections of MPTP of 25 mg/kg per day for five continuous days, and the same volume of saline is injected as a control. All the animals are sacrificed at week 1, 2, 3, or 4 after the LPS or MPTP injections. The brain samples are collected for the subsequent immunohistochemistry and western blot experiments.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.7683 mL 23.8413 mL 47.6826 mL 119.2066 mL
    5 mM 0.9537 mL 4.7683 mL 9.5365 mL 23.8413 mL
    10 mM 0.4768 mL 2.3841 mL 4.7683 mL 11.9207 mL
    15 mM 0.3179 mL 1.5894 mL 3.1788 mL 7.9471 mL
    20 mM 0.2384 mL 1.1921 mL 2.3841 mL 5.9603 mL
    25 mM 0.1907 mL 0.9537 mL 1.9073 mL 4.7683 mL
    30 mM 0.1589 mL 0.7947 mL 1.5894 mL 3.9736 mL
    40 mM 0.1192 mL 0.5960 mL 1.1921 mL 2.9802 mL
    50 mM 0.0954 mL 0.4768 mL 0.9537 mL 2.3841 mL
    H2O 60 mM 0.0795 mL 0.3974 mL 0.7947 mL 1.9868 mL
    80 mM 0.0596 mL 0.2980 mL 0.5960 mL 1.4901 mL
    100 mM 0.0477 mL 0.2384 mL 0.4768 mL 1.1921 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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