OVA Peptide (323-339)

Name: OVA Peptide (323-339)
Brand: MedChemExpress
SKU: HY-P0286
Rating: 5.0 (9 reviews)

製品番号: HY-P0286 純度: 99.98%: Data Sheet SDS COA 取扱説明書
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OVA Peptide (323-339) represents a T and B cell epitope of Ovalbumin (Ova), which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

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OVA Peptide (323-339) 構造式

CAS 番号 : 92915-79-2

容量	価格（税別）	在庫状況	数量
1 mg	$50	在庫あり	Estimated Time of Arrival: December 31
5 mg	$120	在庫あり	Estimated Time of Arrival: December 31
10 mg	$200	在庫あり	Estimated Time of Arrival: December 31
25 mg	$380	在庫あり	Estimated Time of Arrival: December 31
50 mg	$600	在庫あり	Estimated Time of Arrival: December 31
100 mg	$960	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

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純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

OVA Peptide (323-339) represents a T and B cell epitope of Ovalbumin (Ova), which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice.

体外実験

When pulsed with 0.01 μM of OVA Peptide (323-339), M2-expressing B cells lead to an increase in the number of T_H cells mobilizing calcium, compared to M2Y-expressing B cells. To assess if M2-expressing B cells are also able to promote stronger individual responses, we quantified the 405/530 ratio MFI of responding T_H cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

OVA Peptide (323-339) represents a T and B cell epitope of OVA, which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice. Daily aerosolization of OVA Peptide (323-339) for 20 minutes over a period of 10 days has been as effective in the stimulation of a serum anti-OVA IgE antibody response as sensitization to native OVA by the same route. After sensitization to native OVA, the majority of the IgE anti-OVA response is directed against OVA Peptide (323-339). Evaluated by hematoxylin/eosin and major basic protein immunohistochemical stainings, OVA and OVA Peptide (323-339) induce similar lung inflammation. Interestingly, significant serum total IgE and OVA-specific IgE are observed in OVA mice when compared to saline control. OVA Peptide (323-339) mice show higher serum OVA-specific IgE, OVA Peptide (323-339)-specific IgE, IL-4 and lower IFN-γ similar to OVA mice. The proliferative response to OVA is found in cultured splenocytes of both OVA and OVA Peptide (323-339) mice, while the similar proliferative response to OVA Peptide (323-339) is only observed in the splenocytes of OVA Peptide (323-339)-sensitized and challenged mice. Although OVA Peptide (323-339) induces a Th2-like response in the mouse model as does OVA, OVA Peptide (323-339) has clearly limited immunogenic potency to activate OVA-sensitized and challenged mice splenocytes, unlike OVA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1773.90

分子式

C₇₄H₁₂₀N₂₆O₂₅

CAS 番号

92915-79-2

Appearance

Solid

Color

White to off-white

Sequence

Ile-Ser-Gln-Ala-Val-His-Ala-Ala-His-Ala-Glu-Ile-Asn-Glu-Ala-Gly-Arg

Sequence Shortening

ISQAVHAAHAEINEAGR

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

polypeptide, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

H₂O : 10.53 mg/mL (5.94 mM; adjust pH to 3 with CH3COOH)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.5637 mL	2.8186 mL	5.6373 mL
5 mM	0.1127 mL	0.5637 mL	1.1275 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

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体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.98%

Select Batch:

データシート (280 KB) SDS (254 KB)

COA (246 KB) RP-HPLC (250 KB) MS (70 KB) Elemental Analysis Report (106 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540. [Content Brief]

[2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35. [Content Brief]

細胞実験 ^[1]	A20 B cell lines are loaded, or not, with different concentrations of OVA Peptide (323-339) overnight. Cells are then conjugated with purified mouse CD4⁺ T cells and incubated for 20 h. After the 20 h incubation period, supernatant is recovered and stored at -20°C. IFN-γ production is quantified in the supernatants by sandwich ELISA. 96-well plates are analyzed. T cells cultured for two to four days in the presence of 3 μg/mL of anti-CD3 antibody are used as positive control of T cell activation. B cells cultured for 48 h in the presence of 2.5 μg/mL of anti-CD40 antibody and 5 μg/mL of the F(ab’)2/F(ab) portion of an anti-mouse IgG antibody are used as positive control for B cell activation^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[2]	Mice^[2] Fifty-one 8-week-old female BALB/c mice are randomly divided into three groups: OVA, OVA 323-339 and saline. They are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum or saline on days 0, 7, 14. On days 21-23, all groups are challenged intranasally with 20 μL of 1% OVA, 1% OVA Peptide (323-339) and saline, respectively. On days 0, 7, 14, mice are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum, or saline; on days 21-23, all groups are challenged intranasally with either 20 μL of 1% OVA, 1% OVA Peptide (323-339) or saline. On day 28, after killing, splenocytes are isolated and cultured under the stimulus of each allergen or medium. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540. [Content Brief] [2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.5637 mL	2.8186 mL	5.6373 mL	14.0932 mL
H₂O	5 mM	0.1127 mL	0.5637 mL	1.1275 mL	2.8186 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.