Phenol Red sodium salt
Based on 1 publication(s) in Google Scholar
Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye. Phenol Red sodium salt is also an antagonist of rat P2X1R. Phenol Red sodium salt enhances the halogenating activity of myeloperoxidase. Phenol Red sodium salt shows a distinct color change from pink to yellow in a positive pH reaction.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 34487-61-1
- Formula: C19H13NaO5S
- Molecular Weight:376.36
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Phenol Red sodium salt
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Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Phenol Red sodium salt acts as an indicator of AChE activity[4]
1.Solutions Preparation
Concentration of Phenol Red sodium salt in water is set to 500 µM.
2. Reaction
1) Thirty-five microliters of PBS, 25 µL of PBS 7.4 or tacrine solution, 20 µL of Phenol Red sodium salt, and 20 µL of 5 mM AChCl are consequently added to the surface of paper with immobilized AChE.
2) After 5 min of incubation, the surface of the paper is photographed using a smart phone camera placed on a 3D-printed, tube-shaped holder.
Note: in neutral pH of PBS 7.4, Phenol red gives a red color. After addition of AChCl, immediately forming acetic acid acidifying medium and the Phenol Red sodium salt turns yellow.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 34487-61-1
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Appearance Solid
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Molecular Weight 376.36
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Formula C19H13NaO5S
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Color Red to black
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SMILES
O=S1(OC(C2=CC=C(O[Na])C=C2)(C3=CC=C(O)C=C3)C4=CC=CC=C41)=O
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Synonyms
Phenolsulfonephthalein sodium salt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
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STAR Protoc
Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafish. [Abstract]2024 Jun 26;5(3):103156. PMID: 38941183
Solvent & Solubility
DMSO : 5 mg/mL (13.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Morgan A, et al. Caution for the routine use of phenol red - It is more than just a pH indicator. Chem Biol Interact. 2019;310:108739. [Content Brief]
[2]. Peltzer D, et al. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. J Virol Methods. 2021;289:114041. [Content Brief]
[3]. King BF, et al. Antagonism of ATP responses at P2X receptor subtypes by the pH indicator dye, Phenol red. Br J Pharmacol. 2005 Jun;145(3):313-22. [Content Brief]
[4]. Kostelnik A, et al. Color Change of Phenol Red by Integrated Smart Phone Camera as a Tool for the Determination of Neurotoxic Compounds. Sensors (Basel). 2016 Sep 7;16(9):1212. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6570 mL | 13.2852 mL | 26.5703 mL | 66.4258 mL |
| 5 mM | 0.5314 mL | 2.6570 mL | 5.3141 mL | 13.2852 mL | |
| 10 mM | 0.2657 mL | 1.3285 mL | 2.6570 mL | 6.6426 mL |
Phenol Red sodium salt Related Classifications
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