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  4. Phenol Red sodium salt

Phenol Red sodium salt  (Synonyms: Phenolsulfonephthalein sodium salt)

Cat. No.: HY-D0169A Purity: ≥97.0%
COA Handling Instructions

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Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction .

For research use only. We do not sell to patients.

Phenol Red sodium salt Chemical Structure

Phenol Red sodium salt Chemical Structure

CAS No. : 34487-61-1

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Solid + Solvent
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Phenol Red sodium salt:

Phenol Red sodium salt Related Antibodies

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Description

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction[1] [2].

Molecular Weight

376.36

Formula

C19H13NaO5S
CAS No.
Appearance

Solid

Color

Red to black

SMILES

O=S1(OC(C2=CC=C(O[Na])C=C2)(C3=CC=C(O)C=C3)C4=CC=CC=C41)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6570 mL 13.2852 mL 26.5703 mL
5 mM 0.5314 mL 2.6570 mL 5.3141 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥97.0%

COA (261 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1][2]

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6570 mL 13.2852 mL 26.5703 mL 66.4258 mL
5 mM 0.5314 mL 2.6570 mL 5.3141 mL 13.2852 mL
10 mM 0.2657 mL 1.3285 mL 2.6570 mL 6.6426 mL
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Phenol Red sodium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenol Red34487-61-1PhenolsulfonephthaleinFluorescent DyepH indicatorhydrogen peroxideInhibitorinhibitorinhibit

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