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Phenol Red sodium salt  (Synonyms: Phenolsulfonephthalein sodium salt)

Cat. No.: HY-D0169A Purity: ≥97.0%
COA Handling Instructions

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction .

For research use only. We do not sell to patients.

Phenol Red sodium salt Chemical Structure

Phenol Red sodium salt Chemical Structure

CAS No. : 34487-61-1

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10 mM * 1 mL in DMSO USD 55 In-stock
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10 mM * 1 mL
ready for reconstitution
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500 mg USD 50 In-stock
5 g USD 55 In-stock
25 g USD 90 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenol Red sodium salt:

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

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Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction[1] [2].

Molecular Weight









Room temperature in continental US; may vary elsewhere.


4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.29 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6570 mL 13.2852 mL 26.5703 mL
5 mM 0.5314 mL 2.6570 mL 5.3141 mL
10 mM 0.2657 mL 1.3285 mL 2.6570 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: ≥97.0%

Cell Assay

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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