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Phenol Red  (Synonyms: Phenolsulfonephthalein)

Cat. No.: HY-D0169 Purity: 98.0%
Handling Instructions Technical Support

Phenol red (Phenolsulfonephthalein) is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction .

For research use only. We do not sell to patients.

Phenol Red

Phenol Red Chemical Structure

CAS No. : 143-74-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 g In-stock
25 g In-stock
50 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenol Red:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Phenol Red

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Phenol red (Phenolsulfonephthalein) is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction[1] [2].

In Vitro

Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. Phenol red has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

354.38

Formula

C19H14O5S

CAS No.
Appearance

Solid

Color

Red to reddish brown

SMILES

O=S1(OC(C2=CC=C(O)C=C2)(C3=CC=C(O)C=C3)C4=CC=CC=C41)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (141.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8218 mL 14.1091 mL 28.2183 mL
5 mM 0.5644 mL 2.8218 mL 5.6437 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8218 mL 14.1091 mL 28.2183 mL 70.5457 mL
5 mM 0.5644 mL 2.8218 mL 5.6437 mL 14.1091 mL
10 mM 0.2822 mL 1.4109 mL 2.8218 mL 7.0546 mL
15 mM 0.1881 mL 0.9406 mL 1.8812 mL 4.7030 mL
20 mM 0.1411 mL 0.7055 mL 1.4109 mL 3.5273 mL
25 mM 0.1129 mL 0.5644 mL 1.1287 mL 2.8218 mL
30 mM 0.0941 mL 0.4703 mL 0.9406 mL 2.3515 mL
40 mM 0.0705 mL 0.3527 mL 0.7055 mL 1.7636 mL
50 mM 0.0564 mL 0.2822 mL 0.5644 mL 1.4109 mL
60 mM 0.0470 mL 0.2352 mL 0.4703 mL 1.1758 mL
80 mM 0.0353 mL 0.1764 mL 0.3527 mL 0.8818 mL
100 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.7055 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phenol Red
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