Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafish

  • STAR Protoc. 2024 Jun 26;5(3):103156. doi: 10.1016/j.xpro.2024.103156.
Zi-Ling Qu  1 ,  Yi-Bing Zhang  2
Affiliations
  • 1. Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, China. Electronic address: [email protected].
  • 2. Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, China. Electronic address: [email protected].
PMID: 38941183 DOI: 10.1016/j.xpro.2024.103156
Abstract

A gene-rescue experiment under a mutant background is essential to clarify gene function and the resulting biological potential in vivo. Here, we present a protocol for determining the change in interferon response by microinjecting plasmids into one-cell-stage zebrafish embryos. We describe steps for comparing the resistance potential to virus Infection in wild-type and knockout zebrafish larvae following plasmid microinjection. We then detail how to link the enhanced interferon immunity to the improved resistance in knockout zebrafish larvae by gene-rescue experiments. For complete details on the use and execution of this protocol, please refer to Qu et al.1.

Keywords
CRISPR; Genetics; Immunology; Model Organisms; Molecular Biology; Molecular/Chemical Probes.
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