2. Stem Cell/Wnt
  3. Porcupine
  4. Porcn-IN-1

Porcn-IN-1 is potent porcupine inhibitor with an IC50 of 0.5±0.2 nM.

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Porcn-IN-1

Porcn-IN-1 Estructura química

No. CAS : 2036044-77-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
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Porcn-IN-1 Related Antibodies

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Descripciòn

Porcn-IN-1 is potent porcupine inhibitor with an IC50 of 0.5±0.2 nM.

IC50 & Target

IC50: 0.5±0.2 nM (Porcupine inhibitor)[1]
Cellular Effect
Cell Line Type Value Description References
HEK293 IC50
0.5 nM
Compound: 53
Inhibition of porcupine in mouse L Wnt3A cells co-cultured with HEK293 cells after 48 hrs by Super-top flash reporter gene assay
Inhibition of porcupine in mouse L Wnt3A cells co-cultured with HEK293 cells after 48 hrs by Super-top flash reporter gene assay
[PMID: 27692509]
In Vitro

Porcupine is an enzyme that catalyses the addition of palmitoleate to a serine residue in Wnt proteins, a process which is required for the secretion of Wnt proteins. Porcupine-IN-1 is as potent as the clinical compound LGK974 in a cell based STF reporter gene assay. Porcn-IN-1 potently inhibits the secretion of Wnt3A, therefore is confirmed to be a porcupine inhibitor[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Porcn-IN-1 Related Antibodies
In Vivo

Porcn-IN-1 demonstrates moderate clearance under the treatment of human liver microsomes (57 mL/min/kg) and rat liver microsomes (24 mL/min/kg). It exhibits high clearance when treated with mouse microsomes (109 mL/min/kg)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Peso molecular

410.44

Fòrmula

C25H19FN4O
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

FC1=C(C2=CC(C)=NC=C2)N=CC(CNC(C3=CC=C(C(C=CC=C4)=C4N5)C5=C3)=O)=C1
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvente y solubilidad
In Vitro: 

DMSO : ≥ 100 mg/mL (243.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4364 mL 12.1820 mL 24.3641 mL
5 mM 0.4873 mL 2.4364 mL 4.8728 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 99.57%

SDS (393 KB)

COA (247 KB) LCMS (88 KB)

Instrucciones de manejo (2659 KB)
Referencias
Ensayo celular
[1]

HEK293T cells are transfected with pLinbin-Wnt3A plasmid or vehicle control. The HEK293T cells are then treated with or without compounds (Porcn-IN-1). Western Blot is used after 48 h to analyze both the cell lysis and culture medium[1].

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4364 mL 12.1820 mL 24.3641 mL 60.9102 mL
5 mM 0.4873 mL 2.4364 mL 4.8728 mL 12.1820 mL
10 mM 0.2436 mL 1.2182 mL 2.4364 mL 6.0910 mL
15 mM 0.1624 mL 0.8121 mL 1.6243 mL 4.0607 mL
20 mM 0.1218 mL 0.6091 mL 1.2182 mL 3.0455 mL
25 mM 0.0975 mL 0.4873 mL 0.9746 mL 2.4364 mL
30 mM 0.0812 mL 0.4061 mL 0.8121 mL 2.0303 mL
40 mM 0.0609 mL 0.3046 mL 0.6091 mL 1.5228 mL
50 mM 0.0487 mL 0.2436 mL 0.4873 mL 1.2182 mL
60 mM 0.0406 mL 0.2030 mL 0.4061 mL 1.0152 mL
80 mM 0.0305 mL 0.1523 mL 0.3046 mL 0.7614 mL
100 mM 0.0244 mL 0.1218 mL 0.2436 mL 0.6091 mL
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Porcn-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Porcn-IN-12036044-77-4PorcupineInhibitorinhibitorinhibit

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Nombre del producto:
Porcn-IN-1
Cat. No.:
HY-111472
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