2. Cell Cycle/DNA Damage
  3. RAD51
  4. RI-1

RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells.

For research use only. We do not sell to patients.

CAS No. : 415713-60-9

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

RI-1 Related Antibodies

Top Publications Citing Use of Products

    RI-1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Aug 1:512:1-14.  [Abstract]

    The colony formation survival rate of the RPE1 cells was quantitatively analyzed, and the effect of RAD51 (RI-1) dose was examined.

    RI-1 purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Apr 24;21(6):533-544.   [Abstract]

    MDA-MB-157 cells treated with different concentrations of ABT888 for 72 hours in combination with DMSO or a constant dose of 25-50 μM RI-1.

    RI-1 purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Apr 24;21(6):533-544.   [Abstract]

    RI-1 (50 mg/kg). The expression of RAD51 in these four groups of mice was analyzed by immunofluorescence.

    RI-1 purchased from MedChemExpress. Usage Cited in: Hum Mol Genet. 2019 Oct 15;28(20):3422-3430.  [Abstract]

    The PBE rate of oocytes in the control group and the RI-1 (60 μM) treatment group was measured 11 to 16 hours after in vitro maturation.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells[1].

    IC50 & Target

    IC50: 5-30 μM (RAD51)[1]
    In Vitro

    RI-1 (1-50 μM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells[1].
    RI-1 (5-20 μM; 30 min) inhibits HsRAD51 in a concentration-dependent manner[1].
    RI-1 (20 μM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts[1].
    RI-1 (15-25 μM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    RI-1 Related Antibodies
    In Vivo

    RI-1 (50 mg/kg; i.p. every 3 d for 30 d) significantly reduces triple negative breast cancer (TNBC) tumor growth in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female BALB/c nude mice (6 weeks) bearing TNBC tumor[2]
    Dosage: 50 mg/kg
    Administration: I.p. every 3 days for 30 days
    Result: Resulted in significant inhibition of tumor growth.
    Did not cause body weight loss significantly.
    Molecular Weight

    361.61

    Formula

    C14H11Cl3N2O3
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C(C(N1CCOCC1)=C2Cl)N(C3=CC=C(Cl)C(Cl)=C3)C2=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (92.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7654 mL 13.8271 mL 27.6541 mL
    5 mM 0.5531 mL 2.7654 mL 5.5308 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.83%

    SDS (393 KB)

    COA (257 KB) HNMR (257 KB) LCMS (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7654 mL 13.8271 mL 27.6541 mL 69.1353 mL
    5 mM 0.5531 mL 2.7654 mL 5.5308 mL 13.8271 mL
    10 mM 0.2765 mL 1.3827 mL 2.7654 mL 6.9135 mL
    15 mM 0.1844 mL 0.9218 mL 1.8436 mL 4.6090 mL
    20 mM 0.1383 mL 0.6914 mL 1.3827 mL 3.4568 mL
    25 mM 0.1106 mL 0.5531 mL 1.1062 mL 2.7654 mL
    30 mM 0.0922 mL 0.4609 mL 0.9218 mL 2.3045 mL
    40 mM 0.0691 mL 0.3457 mL 0.6914 mL 1.7284 mL
    50 mM 0.0553 mL 0.2765 mL 0.5531 mL 1.3827 mL
    60 mM 0.0461 mL 0.2305 mL 0.4609 mL 1.1523 mL
    80 mM 0.0346 mL 0.1728 mL 0.3457 mL 0.8642 mL
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    RI-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    RI-1415713-60-9RI1RI 1RAD51homologousrecombinationInhibitorinhibitorinhibit

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