2. Antibody-drug Conjugate/ADC Related
  3. Drug-Linker Conjugates for ADC
  4. SMCC-DM1

SMCC-DM1  (Synonyms: DM1-SMCC)

製品番号: HY-101070 純度: 99.23%
COA 取扱説明書 Technical Support

SMCC-DM1 (DM1-SMCC) is a agent-linker conjugate composed of a potent microtubule-disrupting agent DM1 and a linker SMCC to make antibody agent conjugate (ADC).

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CAS 番号 : 1228105-51-8

容量 価格（税別） 在庫状況 数量
1 mg $120 在庫あり
5 mg $180 在庫あり
10 mg $330 在庫あり
25 mg $750 在庫あり
50 mg $1200 在庫あり
100 mg $1680 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 12 publication(s) in Google Scholar

SMCC-DM1 関連抗体

Top Publications Citing Use of Products

    SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Mol Ther. 2022 Jul 6;30(7):2522-2536.  [Abstract]

    ScFab Pigbak#2 and SMCC-DM1 were mixed in a molar ratio of 1:10 in PBS (pH 7.5) at 4 ℃ overnight. B16F10 cells were incubated with Pigbak#2-DM1 (0.05-50 nM) at various concentrations.

    SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.

    SMCC-DM1 (10 mM). Dose-response analysis of the effect of cetuximab-DM1 (1-100000 nM; 120 h) on the proliferation of parental HCC1954 and HCC-TDM1R cells. IC50 values were represented in the graph and detailed in the table.

    SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.

    SMCC-DM1 (10 mM). In vivo effect of cetuximab-DM1 (i.p.; 15 mg/kg) on tumor growth in BALB/c nu/nu mice injected with HCC1954, HCC-TDM1R#19, or HCCTDM1R#20 cells.

    SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.

    SMCC-DM1 (10 mM). Colocalization (yellow) between cetuximab-DM1 (cet-DM1) (30 min-24 h; 10 nM) and LAMP1 occurred at the indicated incubation times in HCC1954 and HCC-TDM1R cells. Cetuximab-DM1 staining red, LAMP1: green, DAPI: blue.

    SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.

    SMCC-DM1 (10 mM). Western blot analyses showed the effect of cetuximab-DM1 (10 nM; 1-3 d) on the EGFR levels in HCC1954 and HCC-TDM1R cells.

    Drug-Linker Conjugates for ADC アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Auristatin Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents Dual payloads

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    SMCC-DM1 (DM1-SMCC) is a agent-linker conjugate composed of a potent microtubule-disrupting agent DM1 and a linker SMCC to make antibody agent conjugate (ADC)[1].

    IC50 & Target

    Maytansinoids

     

    体外実験

    SMCC-DM1 inhibits HCC1954 and MDA-MB-468 cells proliferation with IC50s of 17.2 and 49.9 nM, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SMCC-DM1 関連抗体
    分子量

    1072.61

    分子式

    C51H66ClN5O16S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O[C@H]([C@@]1(C)[C@H]([C@@H]([C@@]2([H])OC(N[C@]3(O)C2)=O)C)O1)CC(N(C)C4=CC(C/C(C)=C/C=C/[C@H]3OC)=CC(OC)=C4Cl)=O)[C@@H](N(C(CCSC(C5=O)CC(N5CC6CCC(C(ON7C(CCC7=O)=O)=O)CC6)=O)=O)C)C
    輸送条件

    Shipping with dry ice.
    保管条件

    -80°C, stored under nitrogen
    溶剤 & 溶解度
    体外: 

    DMSO : 16.67 mg/mL (15.54 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9323 mL 4.6615 mL 9.3231 mL
    5 mM 0.1865 mL 0.9323 mL 1.8646 mL
    10 mM 0.0932 mL 0.4662 mL 0.9323 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.67 mg/mL (1.56 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 1.67 mg/mL (1.56 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.23%

    COA (258 KB) HNMR (451 KB) LCMS (93 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.9323 mL 4.6615 mL 9.3231 mL 23.3076 mL
    5 mM 0.1865 mL 0.9323 mL 1.8646 mL 4.6615 mL
    10 mM 0.0932 mL 0.4662 mL 0.9323 mL 2.3308 mL
    15 mM 0.0622 mL 0.3108 mL 0.6215 mL 1.5538 mL
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    • Your name will appear on the site.

    SMCC-DM1 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SMCC-DM11228105-51-8DM1-SMCCDrug-Linker Conjugates for ADCInhibitorinhibitorinhibit

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    製品名:
    SMCC-DM1
    製品番号:
    HY-101070
    数量:
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