TDCPP
Based on 11 publication(s) in Google Scholar
TDCPP is a chlorinated analog of tris(2,3-dibromopropyl)phosphate (Tris) which is one of the most detected organophosphorus flame retardants (OPFRs) in the environment.
For research use only. We do not sell to patients.
- Purity: 99.71%
- CAS No.: 13674-87-8
- Formula: C9H15Cl6O4P
- Molecular Weight:430.90
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TDCPP
More- J Hazard Mater. 2022 Jul 15;434:128824. [Abstract]
- Environ Int. 2026 Jun 15:214:110373. [Abstract]
- Chemosphere. 2022 Dec;308(Pt 2):136345. [Abstract]
- Sci Total Environ. 2025 Jan 10:961:178429. [Abstract]
- Environ Pollut. 2024 Apr 15:347:123740. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- J Cereb Blood Flow Metab. 2024 Jun 9:271678X241260100. [Abstract]
- Neurochem Res. 2024 Sep;49(9):2453-2468. [Abstract]
- Food Chem Toxicol. 2026 Jul:213:116103. [Abstract]
- Reprod Toxicol. 2026 May 28:144:109269. [Abstract]
- Gene. 2022 May 15:822:146349. [Abstract]
Biological Activity
Exposure to TDCPP does not significantly affect cell viability until at concentration >68 μg/mL. HCECs show a 16% cell viability loss after exposing to 136 μg/mL TDCPP. Moreover, TDCPP induces a sharp decrease in viable cells (87%) after exposing to ≥272 μg/mL TDCPP. Based on cell viability, the LC50 value for TDCPP is 202 μg/mL using a nonlinear regression. Compare to controls, TDCPP-exposed cells exhibit a concentration-dependent increase in apoptosis. Anti-apoptotic Bcl-2 protein expression is increased to 1.4 fold after exposing to 2 μg/mL TDCPP, 1.2-folds at 20 μg/mL but dynamically decreased to 0.4 fold at 200 μg/mL compare to control. The caspase-3 activity is increased to 2.1 folds of the control at 200 μg/mL TDCPP[1]. TDCPP inhibits cell growth at lower concentrations (IC50 of 27 μM), while cell viability and toxicity are affected at higher concentrations (IC50 of 171 μM and 168 μM, respectively)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13674-87-8
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Appearance Liquid (Density: 1.512 g/cm3)
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Molecular Weight 430.90
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Formula C9H15Cl6O4P
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Color Colorless to light yellow
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SMILES
O=P(OC(CCl)CCl)(OC(CCl)CCl)OC(CCl)CCl
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Synonyms
Tris(1,3-dichloroisopropyl)phosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (11)
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Journal Impact Factor
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Most Recent
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J Hazard Mater
Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses. [Abstract]2022 Jul 15;434:128824. PMID: 35427976 -
Environ Int
Integrated network toxicology and transcriptomics reveal NF-κB signaling as a key mediator of TDCPP-induced inflammatory responses in human microglia. [Abstract]2026 Jun 15:214:110373. PMID: 42314475 -
Chemosphere
Organophosphorus flame retardant TDCPP induces neurotoxicity via mitophagy-related ferroptosis in vivo and in vitro. [Abstract]2022 Dec;308(Pt 2):136345. PMID: 36087716 -
Sci Total Environ
TDCPP promotes apoptosis and inhibits the calcium signaling pathway in human neural stem cells. [Abstract]2025 Jan 10:961:178429. PMID: 39798459 -
Environ Pollut
TDCPP and TiO2 NPs aggregates synergistically induce SH-SY5Y cell neurotoxicity by excessive mitochondrial fission and mitophagy inhibition. [Abstract]2024 Apr 15:347:123740. PMID: 38462198 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Cereb Blood Flow Metab
Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic stroke. [Abstract]2024 Jun 9:271678X241260100. PMID: 38853430 -
Neurochem Res
The Role and Mechanism of Ambra1-Mediated Mitophagy in TDCPP-Exposed Mouse Hippocampal Neurons. [Abstract]2024 Sep;49(9):2453-2468. PMID: 38850437 -
Food Chem Toxicol
TDCIPP induces placental dysfunction and fetal growth restriction via oxidative stress-mediated PINK1/Parkin mitophagy. [Abstract]2026 Jul:213:116103. PMID: 42001928 -
Reprod Toxicol
Long-term and low-dose exposure to TDCPP reduces reproductive capacity by enhancing germline apoptosis associated with activation of DNA damage in Caenorhabditis elegans. [Abstract]2026 May 28:144:109269. PMID: 42214638 -
Gene
Transcriptomic investigation of the effects of TDCPP on PC12 and GC2 cells with experimental validation. [Abstract]2022 May 15:822:146349. PMID: 35182677
Solvent & Solubility
Ethanol : 100 mg/mL (232.07 mM; Need ultrasonic)
DMSO : ≥ 62.5 mg/mL (145.05 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 33.33 mg/mL (77.35 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 33.33 mg/mL (77.35 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
The cellular ATP contents are determined in HCECs grown in DMEM containing 0, 2, 20, or 200 μg/mL TDCPP using a luciferase-based ATP assay kit according to the manufacturer's guideline. Briefly, after 24 h exposure, HCECs are lysed with lysis buffer. Lysates are then centrifuged at 12,000 g at 4°C for 5 min. Then, 100 μL of supernatant is mixed with 100 μL ATP detection working dilution. Luminance is examined by an fluorescence microplate reader[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
To examine the effects of TDCPP on cell viability, HCECs are planted into 96-well plate (100 μL/well) at density of 1×105 cells/mL overnight. Then, the medium is changed into fresh medium containing 0.034, 0.34, 3.4, 34, 68, 136, 272, or 340 μg/mL of TDCPP and solvent vehicle (0.1%, v/v) and incubated for 24 h. Cell viability is detected using CCK-8 cell viability assay kit according to the manufacturer's instructions. After exposure, cellular morphology is observed and recorded by an inverted microscopy[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (622 KB)
- English - EN (622 KB)
- Français - FR (622 KB)
- Deutsch - DE (622 KB)
- Norwegian - NO (622 KB)
- Español - ES (622 KB)
- Swedish - SV (622 KB)
- Italian - IT (622 KB)
- Korean - KR (622 KB)
- Portuguese - PT (622 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiang P, et al. Effects of organophosphorus flame retardant TDCPP on normal human corneal epithelial cells: Implications for human health. Environ Pollut. 2017 Nov;230:22-30. [Content Brief]
[2]. Killilea DW, et al. Flame retardant tris(1,3-dichloro-2-propyl)phosphate (TDCPP) toxicity is attenuated by N-acetylcysteine in human kidney cells. Toxicol Rep. 2017 May 17;4:260-264. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 2.3207 mL | 11.6036 mL | 23.2072 mL | 58.0181 mL |
| 5 mM | 0.4641 mL | 2.3207 mL | 4.6414 mL | 11.6036 mL | |
| 10 mM | 0.2321 mL | 1.1604 mL | 2.3207 mL | 5.8018 mL | |
| 15 mM | 0.1547 mL | 0.7736 mL | 1.5471 mL | 3.8679 mL | |
| 20 mM | 0.1160 mL | 0.5802 mL | 1.1604 mL | 2.9009 mL | |
| 25 mM | 0.0928 mL | 0.4641 mL | 0.9283 mL | 2.3207 mL | |
| 30 mM | 0.0774 mL | 0.3868 mL | 0.7736 mL | 1.9339 mL | |
| 40 mM | 0.0580 mL | 0.2901 mL | 0.5802 mL | 1.4505 mL | |
| 50 mM | 0.0464 mL | 0.2321 mL | 0.4641 mL | 1.1604 mL | |
| 60 mM | 0.0387 mL | 0.1934 mL | 0.3868 mL | 0.9670 mL | |
| 80 mM | 0.0290 mL | 0.1450 mL | 0.2901 mL | 0.7252 mL | |
| 100 mM | 0.0232 mL | 0.1160 mL | 0.2321 mL | 0.5802 mL |
TDCPP Related Classifications
HY-108712 Related Classifications
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