2. Metabolic Enzyme/Protease
  3. Epoxide Hydrolase
  4. TPPU

TPPU is a soluble epoxide hydrolase (sEH) inhibitor with IC50 values of 37 and 3.7 nM for monkey and human sEH, respectively.

For research use only. We do not sell to patients.

CAS No. : 1222780-33-7

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of TPPU:

TPPU Related Antibodies

Top Publications Citing Use of Products

    TPPU purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 19:e05656.  [Abstract]

    TPPU (20 μM; 24 h) increased EZH2/H3K27Me3 levels but decreased aging protein expression levels in mGCs under oxidative stress conditions.

    TPPU purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 19:e05656.  [Abstract]

    TPPU (20 μM; 24 h) increased 14,15-EET concentration in mGC culture supernatants.

    TPPU purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 19:e05656.  [Abstract]

    TPPU (20 μM; 24 h) pre-treatment alleviated ROS-induced cell senescence in mGCs.

    TPPU purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 19:e05656.  [Abstract]

    The TEM (EM mice treated with TPPU (3 mg/kg; i.g.)) group mGCs showed decreased SA-β-gal activity compared with the EM group mGCs.

    TPPU purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 19:e05656.  [Abstract]

    5,6-EET, 8,9-EET, 11,12-EET, and 14,15-EET levels were increased in mGCs collected from the TEM (EM mice treated with TPPU (3 mg/kg; i.g.)) group compared with those in the EM-mGCs.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TPPU is a soluble epoxide hydrolase (sEH) inhibitor with IC50 values of 37 and 3.7 nM for monkey and human sEH, respectively.

    IC50 & Target

    IC50: 37 nM (Monkey sEH), 3.7 nM (Human sEH)[1]
    Cellular Effect
    Cell Line Type Value Description References
    HUVEC GI50
    > 25 μM
    Compound: 1770, TPPU
    Antiproliferative activity against HUVEC after 72 hrs by MTT assay
    Antiproliferative activity against HUVEC after 72 hrs by MTT assay
    		[PMID: 23726028]
    HepG2 GI50
    > 25 μM
    Compound: 1770, TPPU
    Cytotoxicity against human HepG2 cells by MTT assay
    Cytotoxicity against human HepG2 cells by MTT assay
    		[PMID: 23726028]
    Huh-7 GI50
    > 25 μM
    Compound: 1770, TPPU
    Cytotoxicity against human HuH7 cells by MTT assay
    Cytotoxicity against human HuH7 cells by MTT assay
    		[PMID: 23726028]
    In Vitro

    Soluble epoxide hydrolase inhibitors (sEHIs) possess anti-inflammatory, antiatherosclerotic, antihypertensive and analgesic properties[1]. In the Caco-2 cells permeability assay, TPPU rapidly passes the cell monolayer, suggesting it will have a good intestinal permeability[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TPPU Related Antibodies
    In Vivo

    TPPU is suitable for investigating soluble epoxide hydrolase biology and the role of epoxide-containing lipids in modulating inflammatory diseases. TPPU displays high plasma concentrations when dosed orally at 0.3 mg/kg and drug-like properties. The Cmax increases with dose from 0.3 to 3 mg/kg for TPPU[1]. Following administration in drinking water to rats (0.2, 1, and 5 mg TPPU/L with 0.2% PEG400), TPPU’s blood concentration increases dose dependently within the treatment period to reach an almost steady state after 8 days[2]. The sEH inhibitor, TPPU, shows antidepressant effects in animal models of depression. Expression of sEH protein is increased in the brain of chronically stressed mice and depressed patients. Prophylactic sEH inhibition or sEH-KO results in resilience to repeated social defeat stress, associated with increased BDNF-TrkB signaling in prefrontal cortex and hippocampus of KO mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    359.34

    Formula

    C16H20F3N3O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC1=CC=C(OC(F)(F)F)C=C1)NC2CCN(C(CC)=O)CC2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (231.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7829 mL 13.9144 mL 27.8288 mL
    5 mM 0.5566 mL 2.7829 mL 5.5658 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.15%

    SDS (419 KB)

    COA (243 KB) HNMR (199 KB) LCMS (267 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Cell monolayers that exceeds a resistance of 300 Ω cm-2 are incubated with either 1 μM or 10 μM of TPPU solution in DMSO on the apical side. Medium samples on the apical and basolateral side are collected and frozen immediately after 1, 3 and 6 hours. The apparent permeability coefficient is calculated for t=1 h[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1][2]

    Rats: Water is provided ad libitum containing 0.2% PEG400 with and without TPPU at a concentration of 0.2 mg/L, 1 mg/L and 5 mg/L during the treatment period (8 days). Before (0 h) and after 2 h, 4 h, 8 h, 1 d, 2 d, 4 d and 8 d, 10 μL blood are sampled from the tail vain. The blood is directly mixed with 50 μL deionzed water28 and frozen until analysis. On day 8, the animals are sacrificed by cardiac puncture after anesthesia. Plasma and whole blood are sampled[2].

    Monkeys: TPPU is prepared in 0.3, 1 and 3 mg/kg doses and is administered to four animals with 48 h dosing intervals based on the t1/2 of the compound obtained from the first cassette dosing. Blood is collected from animals at time points 0, 0.25, 0.5, 1, 2, 3, 4, 8, 24 and 48 h after each dosing for analysis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7829 mL 13.9144 mL 27.8288 mL 69.5720 mL
    5 mM 0.5566 mL 2.7829 mL 5.5658 mL 13.9144 mL
    10 mM 0.2783 mL 1.3914 mL 2.7829 mL 6.9572 mL
    15 mM 0.1855 mL 0.9276 mL 1.8553 mL 4.6381 mL
    20 mM 0.1391 mL 0.6957 mL 1.3914 mL 3.4786 mL
    25 mM 0.1113 mL 0.5566 mL 1.1132 mL 2.7829 mL
    30 mM 0.0928 mL 0.4638 mL 0.9276 mL 2.3191 mL
    40 mM 0.0696 mL 0.3479 mL 0.6957 mL 1.7393 mL
    50 mM 0.0557 mL 0.2783 mL 0.5566 mL 1.3914 mL
    60 mM 0.0464 mL 0.2319 mL 0.4638 mL 1.1595 mL
    80 mM 0.0348 mL 0.1739 mL 0.3479 mL 0.8696 mL
    100 mM 0.0278 mL 0.1391 mL 0.2783 mL 0.6957 mL
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    TPPU Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TPPU1222780-33-7Epoxide HydrolasesEH InhibitorInhibitorinhibitorinhibit

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