2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2763
    BP Fluor 532 maleimide
    BP Fluor 532 maleimide is a photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of BP Fluor 532 is ideally suited for the frequency-doubled Nd:YAG laser line. BP Fluor 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry. Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.
    BP Fluor 532 maleimide
  • HY-D0443
    C.I. Direct black 22
    C.I. Direct black 22 is a good dyeing agent for cotton fabrics.
    C.I. Direct black 22
  • HY-D0107
    Fluorescein dicaproate
    Fluorescein dicaproate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
    Fluorescein dicaproate
  • HY-D0413
    C.I. Acid red 37
    C.I. Acid red 37 is an acid red dye that can be adsorbed and removed by bentone clay.
    C.I. Acid red 37
  • HY-107864R
    Erythrosine B free acid (Standard)
    Erythrosine B free acid (Standard) is the analytical standard of Erythrosine B (free acid) (HY-107864). This product is intended for research and analytical applications. Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation.
    Erythrosine B free acid (Standard)
  • HY-D3315
    Rhodamine B carbohydrazide
    Rhodamine B carbohydrazide is a fluorescent derivative based on Rhodamine B (HY-Y0016) that contains the spirocyclic structure of Rhodamine B; it can be used for the detection of copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals, and nitric oxide (NO).
    Rhodamine B carbohydrazide
  • HY-DY1108
    Nicotinamide 1,N6-ethenoadenine dinucleotide (solution)
    Nicotinamide 1,N6-ethenoadenine dinucleotide (solution) (ε-NAD (solution)) is a fluorescent analog of NAD. Nicotinamide 1,N6-ethenoadenine dinucleotide can be cleaved by phosphodiesterase I (from C. adamanteus venom) and binds to bovine liver glutamate dehydrogenase. Nicotinamide 1,N6-ethenoadenine dinucleotide can serve as a substrate for G-ADP ribosylation of G proteins catalyzed by bacterial toxins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the study of ADP ribosylation reactions.
    Solvent and concentration: ddH2O: 20 mM
    Nicotinamide 1,N6-ethenoadenine dinucleotide (solution)
  • HY-D2424
    Disperse Red 343
    Disperse Red 343 is an azo dye widely used in the textile industry for dyeing polyester fabrics.
    Disperse Red 343
  • HY-D2854B
    FITC-PEG2000-CL
    FITC-PEG2000-CL is a fluorescent dye composed of FITC (HY-66019), PEG and chlorine (-Cl). The chlorine group is an electrophilic active group that can be used for nucleophilic substitution reactions and facilitate subsequent chemical modification (Ex/Em=488/525 nm).
    FITC-PEG2000-CL
  • HY-D0545
    Disperse red 91
    Disperse red 91 is a red dye derived from anthraquinone. It is used in plastics, textiles and cosmetics.
    Disperse red 91
  • HY-115416
    Pigment Red 49:1
    Pigment Red 49:1 (Barium lithol red) is an azo lake pigment. The color of Pigment Red 49:1 ranges from yellow to purplish blue.
    Pigment Red 49:1
  • HY-D0389
    Acid Brown 14
    Acid Brown 14 is a brown dye whose staining effects can be removed by the electrocoagulation (EC) process.
    Acid Brown 14
  • HY-164419C
    FAM labled Atesidorsen sodium
    FAM labled Atesidorsen sodiumis a FAM labled Atesidorsen sodium.
    FAM labled Atesidorsen sodium
  • HY-D0589
    Disperse green 9
    Disperse green 9 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Disperse green 9
  • HY-D0576
    Pigment orange 62
    Pigment orange 62 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Pigment orange 62
  • HY-D0509
    C.I. Acid yellow 172
    C.I. Acid yellow 172 is an acidic yellow dye that can be adsorbed and removed by bentone.
    C.I. Acid yellow 172
  • HY-D2489
    Cy5-tetrazine
    Cy5-tetrazine is a fluorescent dye. Cy5-tetrazine is a CY5 (HY-D0821) derivative contains a tetrazine moiety. Cyanine5.5 tetrazine can be used for in vivo imaging and low background applications. Cy5-tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
    Cy5-tetrazine
  • HY-W327027
    7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one
    7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) is a fluorescent probe for the detection of hydrogen sulfide (H2S). 7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one has a low detection limit (4×10-6 mol/L), good selectivity and high sensitivity. 7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one shows almost no cytotoxicity at concentrations of 150 µg/mL. 7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one has the excitation peak of 331 nm, and the emission peak about 385 nm in DMSO solvent. Upon the addition of increasing amounts of HS-, the fluorescence intensity increases obviously at about 392 nm.
    7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one
  • HY-D0397
    C.I. Direct Violet 9
    C.I. Direct Violet 9 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    C.I. Direct Violet 9
  • HY-D1562
    CytoTrace Red CMTPX
    CytoTrace Red CMTPX is a cell permeable long-term cell tracer (Ex=577 nm, Em=602 nm).
    CytoTrace Red CMTPX
Cat. No. Product Name / Synonyms Application Reactivity