CytoTrace Red CMTPX 

CytoTrace Red CMTPX is a cell permeable long-term cell tracer (Ex=577 nm, Em=602 nm)^[1].

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs)^[2].
1. Preparation of stock solution: Dissolve the dye using high-quality DMSO and prepare a stock solution of 2-10 mM.
2. Preparation of working solution: Dilute the stock solution with preheated serum-free buffer (such as Hanks balanced salt solution (HBSS) containing 20 mM HEPES) to prepare a working solution with a final concentration of 1-20 μM.
3. Staining of adherent cells: (1) Remove the original culture medium.
(2) Add the working solution to the cells.
(3) Incubate at 37℃ for 15 to 45 minutes.
(4) Replace with a fresh, fully grown culture medium.
(5) Before imaging, allow the cells to recover for at least 15-30 minutes to reduce the background signal.
4. Suspension cell staining: Centrifuge the cells to collect them, resuspend them in the working solution, incubate, wash, and then resuspend them in fresh culture medium.
5. Detection: The detection is carried out using a channel with excitation/emission wavelengths of 577/602 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1372.48

C₈₄H₈₀Cl₂N₆O₈

610

589

CN1C2=CC3=[O+]C4=CC5=C(C(C)=CC(C)(C)N5C)C=C4C(C6=C(C([O-])=O)C=C(NC(C7=CC=C(CCl)C=C7)=O)C=C6)=C3C=C2C(C)=CC1(C)C.CN8C9=CC%10=[O+]C%11=CC%12=C(C(C)=CC(C)(C)N%12C)C=C%11C(C%13=C(C([O-])=O)C=CC(NC(C%14=CC=C(CCl)C=C%14)=O)=C%13)=C%10C=C9C(C)=CC8(C)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bernd R Stab 2nd, et al. Mitochondrial Functional Changes Characterization in Young and Senescent Human Adipose Derived MSCs. Front Aging Neurosci. 2016 Dec 15;8:299.  [Content Brief]