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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-123067
    NBD-Fructose
    		99.7%
    NBD-Fructose is a fluorescent derivative of Fructose (HY-N0395) that is formed by coupling NBD-chloride with the amine group of amino fructose (EX/Em=472/538 nm).
    NBD-Fructose
  • HY-DY1036
    Calcein-AM (solution)
    Calcein AM (solution) , has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein) , which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm.
    Solvent and concentration: DMSO: 2 mM
    Calcein-AM (solution)
  • HY-131131
    5-CFDA-AM
    		98.27%
    5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein, which contains an additional negative charge and can be better retained in the cell. 5-CFDA-AM can be used to detect cell viability.
    5-CFDA-AM
  • HY-D0076
    4,5-Diaminofluorescein
    		98.52%
    4,5-Diaminofluorescein is a fluorescent detector for nitric oxide (NO) in cells and tissues.
    4,5-Diaminofluorescein
  • HY-D1091
    DiSBAC2(3)
    		99.87%
    DiSBAC2(3) is an anionic membrane-potential-sensitive dye. DiSBAC2(3) is a plasma membrane potential indicator.
    DiSBAC2(3)
  • HY-101887
    Calcein Blue
    Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator.
    Calcein Blue
  • HY-128448
    Carmoisine
    		98.66%
    Carmoisine (Azorubine) is an azo dye that can be used as a food additive.
    Carmoisine
  • HY-D1252
    Fast-TRFS
    		99.72%
    Fast-TRFS is a selective and superfast fluorogenic probe of thioredoxin reductase (TrxR). Fast-TRFS can be used for imaging TrxR activity in live cells.
    Fast-TRFS
  • HY-15939
    6-FAM SE
    		98.08%
    6-FAM SE (6-carboxyfluorescein succinimidyl ester) is a fluorescent labeling reagent. 6-FAM SE is used for oligonucleotide labeling and DNA sequencing.
    6-FAM SE
  • HY-Y0699
    Methyl red
    		99.55%
    Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry.
    Methyl red
  • HY-W027544
    MCA
    		98.87%
    MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities.
    MCA
  • HY-108715
    Real Thiol
    		98.37%
    Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
    Real Thiol
  • HY-120959
    DAUDA
    		98.08%
    DAUDA (11-(dansylamino) undecanoic acid) is an environment-sensitive fluorescent fatty acid analogue. DAUDA alters its intensities and fluorescent emission spectra on entry into binding proteins. DAUDA is used to determine the relative affinity of natural fatty acids for polymorphs of the Schistosoma mansoni Sm14 fatty acid-binding protein[1].
    DAUDA
  • HY-D0102
    4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole
    		98.75%
    4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) is a fluorescent reagent for the sensitive and specific detection of thiols (Ex/Em: 380/510 nm). 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole has low fluorescence background, and good stability of fluorophor.
    4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole
  • HY-D1092
    DiBAC4(5)
    		99.65%
    DiBaC4(5) is a fluorescent voltage-sensitive dye that can be used to monitor the transmembrane potentials when Papain-dissociated retinal cells from adult zebrafish were exposed to GABAergic ligands. DiBaC4(5) is a potential-sensitive fluorescence dye.
    DiBAC4(5)
  • HY-D1691
    BODIPY-581/591 NHS ester
    		98.67%
    BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester can be used for the labeling of amine containing biomolecules.
    BODIPY-581/591 NHS ester
  • HY-P4419A
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
    		99.24%
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate is a fluorogenic substrate for the determination of protease activity. Boc-Asp(OBzl)-Pro-Arg-AMC acetate undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
  • HY-160116
    α-Synuclein aggregate binder 1
    		98.11%
    α-Synuclein aggregate binder 1 (Compound C05-05) is a specific binder for α-synuclein aggregates and can inhibit α-synuclein aggregation. α-Synuclein aggregate binder 1 can be used as a fluorescent probe (excitation wavelength 900 nm, detection wavelength 500-550 nm) for optical imaging, and can also inhibit α-synuclein fibril formation by blocking the aggregation process. α-Synuclein aggregate binder 1 can be used for positron emission tomography (PET) imaging after being labeled with 18F. α-Synuclein aggregate binder 1 can be used for visual diagnosis of brain lesions and mechanism research of neurodegenerative diseases such as Parkinson's disease and Lewy body dementia.
    α-Synuclein aggregate binder 1
  • HY-D1800
    Vari Fluor 680 SE
    Vari Fluor 680 SE (VF 680 SE) is a dye marker of the Vari Fluor SE series (Ex/Em=680 nm/700 nm). The Vari Fluor SE series of dyes are a class of fluorescent dyes containing NHS ester groups used to label free amines (-NHX) on antibodies, proteins, peptides, amine-modified oligonucleotides and other biomolecules.
    Vari Fluor 680 SE
  • HY-W013967
    1,6-Diphenylhexa-1,3,5-triene
    		98.26%
    1,6-Diphenylhexa-1,3,5-triene is a membrane intercalator and hydrophobic fluorescent probe. 1,6-Diphenylhexa-1,3,5-triene intercalates into the non-polar region of lipid bilayers of adipocyte membranes with probe/membrane equilibrium affected by temperature, probe concentration, and membrane concentration. 1,6-Diphenylhexa-1,3,5-triene’s fluorescence intensity correlating with adipocyte membrane probe incorporation (Ex/Em = 350/452 nm).
    1,6-Diphenylhexa-1,3,5-triene
Cat. No. Product Name / Synonyms Application Reactivity