5-CFDA-AM
Based on 1 Customer Validation
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein, which contains an additional negative charge and can be better retained in the cell. 5-CFDA-AM can be used to detect cell viability.
For research use only. We do not sell to patients.
- Purity: 98.27%
- CAS No.: 124412-00-6
- Formula: C28H20O11
- Molecular Weight:532.45
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
5-CFDA-AM functions as a fluorometric cell viability reagent, where intracellular esterases in viable adherent and suspension cells cleave the compound to generate a measurable fluorescent signal at excitation 493 nm and emission 541 nm, enabling quantification of viable cell populations[1].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM is recommended.
1.2 Preparation of working solution
Dilute to 1-10 μM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell Staining
2.1 Add the working solution and incubate at 37°C for 15-30 minutes.
2.2 Washing and detection: Centrifuge to remove the supernatant, wash with PBS, and then detect using a fluorescence microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 124412-00-6
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Appearance Solid
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Molecular Weight 532.45
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Formula C28H20O11
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Color White to off-white
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SMILES
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL (234.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8781 mL | 9.3906 mL | 18.7811 mL | 46.9528 mL |
| 5 mM | 0.3756 mL | 1.8781 mL | 3.7562 mL | 9.3906 mL | |
| 10 mM | 0.1878 mL | 0.9391 mL | 1.8781 mL | 4.6953 mL | |
| 15 mM | 0.1252 mL | 0.6260 mL | 1.2521 mL | 3.1302 mL | |
| 20 mM | 0.0939 mL | 0.4695 mL | 0.9391 mL | 2.3476 mL | |
| 25 mM | 0.0751 mL | 0.3756 mL | 0.7512 mL | 1.8781 mL | |
| 30 mM | 0.0626 mL | 0.3130 mL | 0.6260 mL | 1.5651 mL | |
| 40 mM | 0.0470 mL | 0.2348 mL | 0.4695 mL | 1.1738 mL | |
| 50 mM | 0.0376 mL | 0.1878 mL | 0.3756 mL | 0.9391 mL | |
| 60 mM | 0.0313 mL | 0.1565 mL | 0.3130 mL | 0.7825 mL | |
| 80 mM | 0.0235 mL | 0.1174 mL | 0.2348 mL | 0.5869 mL | |
| 100 mM | 0.0188 mL | 0.0939 mL | 0.1878 mL | 0.4695 mL |
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