1. Cell Cycle/DNA Damage
    TGF-beta/Smad
    Stem Cell/Wnt
  2. ROCK
  3. Thiazovivin

Thiazovivin 

Cat. No.: HY-13257 Purity: 99.32%
Handling Instructions

Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells.

For research use only. We do not sell to patients.

Thiazovivin Chemical Structure

Thiazovivin Chemical Structure

CAS No. : 1226056-71-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 106 In-stock
Estimated Time of Arrival: December 31
5 mg USD 96 In-stock
Estimated Time of Arrival: December 31
10 mg USD 166 In-stock
Estimated Time of Arrival: December 31
50 mg USD 312 In-stock
Estimated Time of Arrival: December 31
100 mg USD 468 In-stock
Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

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Description

Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells.

IC50 & Target[1]

ROCK

 

In Vitro

Thiazovivin is a ROCK inhibitor. Thiazovivin (2 μM) inhibits ROCK activity and protects human embryonic stem cells (hESCs). Thiazovivin significantly increases the survival of hESCs after dissociation while maintaining pluripotency. Thiazovivin enhances cell-ECM adhesion-mediated integrin signaling. Thiazovivin also stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions[1]. Thiazovivin increases cellular attachment of embryo-derived stem-like cells (eSLCs) of cattle and formation of primary colonies on the feeder layer. Thiazovivin reinforces putative colony outgrowth and supports the expansion of eSLC cultures during the subculture for passaging. Furthermore, Thiazovivin causes greater expression of ectodermal lineage-specific genes in eSLCs of cattle[2].

Molecular Weight

311.36

Formula

C₁₅H₁₃N₅OS

CAS No.

1226056-71-8

SMILES

O=C(C1=CSC(NC2=NC=NC=C2)=N1)NCC3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 30 mg/mL (96.35 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2117 mL 16.0586 mL 32.1172 mL
5 mM 0.6423 mL 3.2117 mL 6.4234 mL
10 mM 0.3212 mL 1.6059 mL 3.2117 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

For cell proliferation assays, embryo-derived stem-like cells (eSLCs) are newly passaged and cultured in 3i system for 48 h on the feeder-free condition to prevent contamination of the BrdU-positive feeder cells. Cells are fixed with 4% paraformaldehyde in PBS (pH 7.4) at 37°C for 2 h, acid-treated with 2 N HCl in PBS for 30 min at 45°C, equilibrated with 0.1 M borate buffer (pH 8.5), and finally incubated with blocking buffer (20% Calf serum; 0.1% Triton X-100; 1% DMSO in PBS) for 2 h. Fixed cells are immunostained with antibodies against anti-BrdU mouse monoclonal antibody IgG followed by incubation with the secondary antibodies FITC conjugated goat anti-mouse IgG. The treated cells are covered with slow-fade anti-fade with DAPI for nuclear staining and covered with a glass coverslip. Images are captured with the fluorescence microscope[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.32%

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Thiazovivin
Cat. No.:
HY-13257
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