Thiazovivin
Based on 12 publication(s) in Google Scholar
Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells. Thiazovivin improves the efficiency of iPSC generation.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 1226056-71-8
- Formula: C15H13N5OS
- Molecular Weight:311.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Kinase Inhibitor Library
HY-L009
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Stem Cell Signaling Compound Library
HY-L017
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TGF-beta/Smad Compound Library
HY-L018
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Anti-Aging Compound Library
HY-L034
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Reprogramming Compound Library
HY-L039
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Cytoskeleton Compound Library
HY-L060
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Cancer Stem Cells Compound Library
HY-L135
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Serine/Threonine Kinase Inhibitor Library
HY-L164
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Extracellular Vesicles (EVs) Compound Library
HY-L168
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Bioactive Compound Library Max
HY-L181
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Anti-Aging Compound Library Mini
HY-L034M
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) Thiazovivin
More- Cell Rep. 2017 Aug 29;20(9):2227-2237. [Abstract]
- Eur J Pharmacol. 2026 Jan 20:1012:178484. [Abstract]
- Sci Rep. 2019 Mar 5;9(1):3462. [Abstract]
- BMC Genomics. 2024 Oct 25;25(1):1001. [Abstract]
- Viruses. 2026 Feb 12;18(2):229. [Abstract]
- bioRxiv. 2026 Jan 15.
- Res Sq. 2025 Nov 10.
- bioRxiv. 2025 Aug 19.
- bioRxiv. 2025 May 4:2025.04.29.651320. [Abstract]
- bioRxiv. 2025 Mar 23:2025.03.23.644821. [Abstract]
- bioRxiv. 2024 Dec 1:2023.12.27.573461. [Abstract]
- Patent. US20180263995A1.
Biological Activity
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ROCK |
Thiazovivin is a ROCK inhibitor. Thiazovivin (2 μM) inhibits ROCK activity and protects human embryonic stem cells (hESCs). Thiazovivin significantly increases the survival of hESCs after dissociation while maintaining pluripotency. Thiazovivin enhances cell-ECM adhesion-mediated integrin signaling. Thiazovivin also stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions[1]. Thiazovivin increases cellular attachment of embryo-derived stem-like cells (eSLCs) of cattle and formation of primary colonies on the feeder layer. Thiazovivin reinforces putative colony outgrowth and supports the expansion of eSLC cultures during the subculture for passaging. Furthermore, Thiazovivin causes greater expression of ectodermal lineage-specific genes in eSLCs of cattle[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1226056-71-8
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Appearance Solid
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Molecular Weight 311.36
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Formula C15H13N5OS
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Color Off-white to light yellow
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SMILES
O=C(C1=CSC(NC2=NC=NC=C2)=N1)NCC3=CC=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (12)
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Journal Impact Factor
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Most Recent
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Cell Rep
Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening. [Abstract]2017 Aug 29;20(9):2227-2237. PMID: 28854370 -
Eur J Pharmacol
Functional in vitro assessment of the HER2 Ile655Val variant on trastuzumab-induced cardiotoxicity in human cardiomyocytes. [Abstract]2026 Jan 20:1012:178484. PMID: 41423083 -
Sci Rep
Suppression of glioblastoma by a drug cocktail reprogramming tumor cells into neuronal like cells. [Abstract]2019 Mar 5;9(1):3462. PMID: 30837577 -
BMC Genomics
Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes. [Abstract]2024 Oct 25;25(1):1001. PMID: 39455925 -
Viruses
ASFV MGF110-7L Inhibits eIF4G1 Expression via Endoplasmic Reticulum Stress to Block Host Translation. [Abstract]2026 Feb 12;18(2):229. PMID: 41754572 -
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bioRxiv
2025 May 4:2025.04.29.651320. PMID: 40654780 -
bioRxiv
CellBouncer, A Unified Toolkit for Single-Cell Demultiplexing and Ambient RNA Analysis, Reveals Hominid Mitochondrial Incompatibilities. [Abstract]2025 Mar 23:2025.03.23.644821. PMID: 40166335 -
bioRxiv
2024 Dec 1:2023.12.27.573461. PMID: 38234800 -
Solvent & Solubility
DMSO : ≥ 30 mg/mL (96.35 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
For cell proliferation assays, embryo-derived stem-like cells (eSLCs) are newly passaged and cultured in 3i system for 48 h on the feeder-free condition to prevent contamination of the BrdU-positive feeder cells. Cells are fixed with 4% paraformaldehyde in PBS (pH 7.4) at 37°C for 2 h, acid-treated with 2 N HCl in PBS for 30 min at 45°C, equilibrated with 0.1 M borate buffer (pH 8.5), and finally incubated with blocking buffer (20% Calf serum; 0.1% Triton X-100; 1% DMSO in PBS) for 2 h. Fixed cells are immunostained with antibodies against anti-BrdU mouse monoclonal antibody IgG followed by incubation with the secondary antibodies FITC conjugated goat anti-mouse IgG. The treated cells are covered with slow-fade anti-fade with DAPI for nuclear staining and covered with a glass coverslip. Images are captured with the fluorescence microscope[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34. [Content Brief]
[2]. Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2117 mL | 16.0586 mL | 32.1172 mL | 80.2929 mL |
| 5 mM | 0.6423 mL | 3.2117 mL | 6.4234 mL | 16.0586 mL | |
| 10 mM | 0.3212 mL | 1.6059 mL | 3.2117 mL | 8.0293 mL | |
| 15 mM | 0.2141 mL | 1.0706 mL | 2.1411 mL | 5.3529 mL | |
| 20 mM | 0.1606 mL | 0.8029 mL | 1.6059 mL | 4.0146 mL | |
| 25 mM | 0.1285 mL | 0.6423 mL | 1.2847 mL | 3.2117 mL | |
| 30 mM | 0.1071 mL | 0.5353 mL | 1.0706 mL | 2.6764 mL | |
| 40 mM | 0.0803 mL | 0.4015 mL | 0.8029 mL | 2.0073 mL | |
| 50 mM | 0.0642 mL | 0.3212 mL | 0.6423 mL | 1.6059 mL | |
| 60 mM | 0.0535 mL | 0.2676 mL | 0.5353 mL | 1.3382 mL | |
| 80 mM | 0.0401 mL | 0.2007 mL | 0.4015 mL | 1.0037 mL |
Thiazovivin Related Classifications
HY-13257 Related Classifications
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