2. Metabolic Enzyme/Protease
  3. Prolyl Endopeptidase (PREP)
  4. Z-321

Z-321 is a prolylendopeptidase (PEP) inhibitor.

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Z-321

Z-321 Estructura química

No. CAS : 130849-58-0

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
50 mg   Obtener un presupuesto  
100 mg   Obtener un presupuesto  

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Z-321 Related Antibodies

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Descripciòn

Z-321 is a prolylendopeptidase (PEP) inhibitor.

IC50 & Target

PEP[1]
In Vivo

Z-321 is a prolylendopeptidase (PEP) inhibitor. In the 100 or 200 mg/kg Z-321-treated groups, mean lordosis quotient (LQ) decreases after administration. However, there is no statistical difference among the values before and after. In contrast, the mean LQ in the 300 mg/kg Z-321-treated females is lower than that before injection (P<0.005). Furthermore, when compare to that in the vehicle-treated control group, mean LQ is also significantly lower (p<0.05). The incidence of soliciting behavior decreases after treatment with 300 mg/kg Z-321, when compare to that before treatment, but there is no statistical difference. The present study also demonstrates that 300 mg/kg Z-321 is effective in inhibiting lordosis behavior without disturbance of locomotor activity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Peso molecular

344.47

Fòrmula

C19H24N2O2S
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1N(C(CC2CC3=C(C=CC=C3)C2)=O)CSC1)N4CCCC4
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 250 mg/mL (725.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9030 mL 14.5151 mL 29.0301 mL
5 mM 0.5806 mL 2.9030 mL 5.8060 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (18.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.25 mg/mL (18.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
Referencias
Administraciòn de animales
[1]

Femal wistar rats (200 to 250 g) are used and housed under conditions of controlled temperature (23 to 26°C) and photoperiod (14: 10 h, light:dark). Fifty four rats are ovariectomized under ether anesthesia. Two weeks after ovariectomy, sexual behavioral tests are carried out before and after a treatment with Z-321 (100, 200 or 300 mg/kg). Another group of rats, 7 females are given 1 mL/kg of 10% gum arabic as control group[1].

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9030 mL 14.5151 mL 29.0301 mL 72.5753 mL
5 mM 0.5806 mL 2.9030 mL 5.8060 mL 14.5151 mL
10 mM 0.2903 mL 1.4515 mL 2.9030 mL 7.2575 mL
15 mM 0.1935 mL 0.9677 mL 1.9353 mL 4.8384 mL
20 mM 0.1452 mL 0.7258 mL 1.4515 mL 3.6288 mL
25 mM 0.1161 mL 0.5806 mL 1.1612 mL 2.9030 mL
30 mM 0.0968 mL 0.4838 mL 0.9677 mL 2.4192 mL
40 mM 0.0726 mL 0.3629 mL 0.7258 mL 1.8144 mL
50 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4515 mL
60 mM 0.0484 mL 0.2419 mL 0.4838 mL 1.2096 mL
80 mM 0.0363 mL 0.1814 mL 0.3629 mL 0.9072 mL
100 mM 0.0290 mL 0.1452 mL 0.2903 mL 0.7258 mL
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Z-321 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-321130849-58-0Z321Z 321Prolyl Endopeptidase (PREP)prolyl oligopeptidasePOPInhibitorinhibitorinhibit

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Nombre del producto:
Z-321
Cat. No.:
HY-19123
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