L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt (Synonyms: L-γ-Glutamyl-3-carboxy-4-nitroanilide ammonium salt)

製品番号: HY-15936 純度: 99.76%: Data Sheet SDS COA 取扱説明書
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L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt is a donor substrate of gamma-glutamyltransferase (GGT) that can be used to measure GGT activity.

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L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt 構造式

CAS 番号 : 63699-78-5

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
25 mg	$50	在庫あり	Estimated Time of Arrival: December 31
50 mg	$70	在庫あり	Estimated Time of Arrival: December 31
100 mg	$95	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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純度とドキュメンテーション
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製品説明

L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt is a donor substrate of gamma-glutamyltransferase (GGT) that can be used to measure GGT activity^[1]^[2].

体外実験

GGT Activity Assay: Purified enzyme Is incubated with L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt (Glu-cp-NA) (1 mM) and Glycylglycine (HY-D0889) (20 mM) in PBS (0.1 M, pH 8.0) at 37°C, and the increase in absorbance at 405 nm is monitored. Enzyme activity is determined using the molar absorptivity of 8800 M^-1cm^-1 formed by p-nitroaniline^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

328.28

分子式

C₁₂H₁₆N₄O₇

CAS 番号

63699-78-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)C1=CC(NC(CC[C@H](N)C(O)=O)=O)=CC=C1[N+]([O-])=O.N

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶剤 & 溶解度

体外:

H₂O : ≥ 30 mg/mL (91.39 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0462 mL	15.2309 mL	30.4618 mL
5 mM	0.6092 mL	3.0462 mL	6.0924 mL
10 mM	0.3046 mL	1.5231 mL	3.0462 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 7.14 mg/mL (21.75 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.76%

Select Batch:

データシート (273 KB) SDS (394 KB)

COA (249 KB) HNMR (267 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Shaw LM, et al. Gamma-Glutamyltransferase: kinetic properties and assay conditions when gamma-glutamyl-4-nitroanilide and its 3-carboxy derivative are used as donor substrates. Clin Chem. 1977 Jan;23(1):79-85. [Content Brief]

[2]. Angeli V, et al. A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism. Arch Biochem Biophys. 2009 Jan 15;481(2):191-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.0462 mL	15.2309 mL	30.4618 mL	76.1545 mL
	5 mM	0.6092 mL	3.0462 mL	6.0924 mL	15.2309 mL
	10 mM	0.3046 mL	1.5231 mL	3.0462 mL	7.6155 mL
	15 mM	0.2031 mL	1.0154 mL	2.0308 mL	5.0770 mL
	20 mM	0.1523 mL	0.7615 mL	1.5231 mL	3.8077 mL
	25 mM	0.1218 mL	0.6092 mL	1.2185 mL	3.0462 mL
	30 mM	0.1015 mL	0.5077 mL	1.0154 mL	2.5385 mL
	40 mM	0.0762 mL	0.3808 mL	0.7615 mL	1.9039 mL
	50 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.5231 mL
	60 mM	0.0508 mL	0.2538 mL	0.5077 mL	1.2692 mL
	80 mM	0.0381 mL	0.1904 mL	0.3808 mL	0.9519 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt Related Classifications

Metabolic Disease

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Glutamic acid γ-(3-carboxy-4-nitroanilide)63699-78-5L-γ-Glutamyl-3-carboxy-4-nitroanilideγ-Glutamyl Transferase (GGT)γ-Glutamyl Transferase, Gamma-Glutamyl Transferaseγ-Glutamyl Transferase SubstrateInhibitorinhibitorinhibit

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