2. Immunology/Inflammation
  3. CD3
  4. Anti-Mouse CD3 Antibody (17A2)

Anti-Mouse CD3 Antibody (17A2) 

Cat. No.: HY-P99124 Pureza: 99%
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Anti-Mouse CD3 Antibody (17A2) is an anti-mouse CD3 IgG2b antibody inhibitor derived from the host Rat. Anti-Mouse CD3 Antibody (17A2) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic T cells. Anti-Mouse CD3 Antibody (17A2) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models.

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Anti-Mouse CD3 Antibody (17A2) Related Antibodies

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1 Publications Citing Use of MCE Anti-Mouse CD3 Antibody (17A2)

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Descripciòn

Anti-Mouse CD3 Antibody (17A2) is an anti-mouse CD3 IgG2b antibody inhibitor derived from the host Rat. Anti-Mouse CD3 Antibody (17A2) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic T cells. Anti-Mouse CD3 Antibody (17A2) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models[1][2].

Isotype

Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity

Mouse
In Vitro

Anti-Mouse CD3 Antibody (17A2) (1-1000 ng/mL, 96 h) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic pan-T cells[1].
Anti-Mouse CD3 Antibody (17A2) (1 μg/mL, 48 h) significantly elevates mRNA levels for these Th1 and IL-17 genes in splenic T cells[1].
Anti-Mouse CD3 Antibody (17A2) (48 h) significantly enhances IRBP (interphotoreceptor retinoid-binding protein)-stimulated production of IFN-γ in ocular cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD3 Antibody (17A2) Related Antibodies

RT-PCR[1]

Cell Line: Splenic T cells
Concentration: 1 μg/mL
Incubation Time: 48 h
Result: Increased the levels of IL-1α, IFN-γ, T-bet and IL-17 and decreased the levels of IL-10 and Foxp3.
In Vivo

Anti-Mouse CD3 Antibody (17A2) (100 or 400 μg, i.v., a single dose) depletes T cells in mice[2].
Anti-Mouse CD3 Antibody (17A2) (200 or 400 μg, i.v., 20 h after irradiation and 4 h before transplantation) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice[2]
Dosage: 100 and 400 μg
Administration: Intravenously injection
Result: Significantly reduced T cells.
Animal Model: Graft-versus-host disease (GVHD) mice models[2]
Dosage: 200 and 400 μg
Administration: Intravenously injection, 20 h after irradiation and 4 h before spleen cells and bone marrow cells transplantation
Result: Suppressed GVHD mortality by about 50% on day 100 posttransplantation and 20% to 40% on day 200.
Prolonged antibody treatment by further injections of 200 μg on days 3,6, and 9 after transplantation.
led to long-term survivors with 90% to 97% chimerism on days 50 and 100.
Reduced CD4+ cells to about 50% and CD8+ cells to 10% after 50 days.
Gene ID

12501  [NCBI]

Accession
Application

in vitro T cell stimulation/activation
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Peso molecular

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD3 Antibody (17A2)]
Formulation

Please refer to the lot-specific COA for specific buffer information.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized CD3E-CD3G Heterodimer Protein, Mouse (HEK293, Fc, HY-P75414) can bind Anti-Mouse CD3 Antibody (17A2). The EC50 for this effect is 31.62 ng/mL.
Pureza y Documentación

Purity: 99%

SDS (251 KB)

COA (232 KB) SDS-PAGE (70 KB) SEC-HPLC (124 KB)

Inhibitory Antibodies User Guide (603 KB)
Referencias
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Anti-Mouse CD3 Antibody (17A2) Related Classifications

  • Calculadora de molaridad

  • Calculadora de dilución

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD3 Antibody (17A2)CD3Cluster of differentiation 3T cellsIFN γIL-17IRBPGVHDInhibitorinhibitorinhibit

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Nombre del producto:
Anti-Mouse CD3 Antibody (17A2)
Cat. No.:
HY-P99124
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