HRP-conjugated Goat Anti-Human IgG H&L

Art. -Nr.: HY-P83662: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

HRP-conjugated Goat Anti-Human IgG H&L is a horseradish peroxidase (HRP) labeled and goat-derived polyclonal antibody, which served as the secondary antibody.

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Größe	Verfügbarkeit	Menge
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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HRP-conjugated Goat Anti-Human IgG H&L Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung
Verweise

Beschreibung

HRP-conjugated Goat Anti-Human IgG H&L is a horseradish peroxidase (HRP) labeled and goat-derived polyclonal antibody, which served as the secondary antibody.

Host

Goat

Clonality

Polyclonal

Species Reactivity

Human

Immunogen

Human IgG.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:20000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	Reinheit	Affinity Purified
Conjugation	HRP	Modification	Unmodified

Appearance

Solution

Formulation

Supplied in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Proclin.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Human IgG-10ng (lane 1, 10 ng), Human IgG-5ng (lane 2, 5 ng), Human IgG-2ng (lane 3, 2 ng), Human IgG-1ng (lane 4, 1 ng), Human IgG-0.5ng (lane 5, 0.5 ng), and Human IgG-0.1ng (lane 6, 0.1 ng) using Goat Anti-Human IgG H&L (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

RRID

AB_3676574

Research Field

Immunology

Dokumentation

Select Batch:

Data Sheet (254 KB) SDS (252 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/u-937-cell.html [Content Brief]

HRP-conjugated Goat Anti-Human IgG H&L Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HRP-conjugated Goat Anti-Human IgG H&L

HRP-conjugated Goat Anti-Human IgG H&L Spezialitätsempfehlungen:

Product Detail

Verification Image

Background

Beschreibung

Verweise

HRP-conjugated Goat Anti-Human IgG H&L Related Classifications

Ihre kürzlich angesehenen Produkte:

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