Bis-ANS dipotassium 

Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a K_d of 2 μM^[1]. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations^[2].

Bis-ANS is a fluorescent molecule that has been used to probe exposed hydrophobic patches in proteins and to monitor the formation of protein aggregates. Bis-ANS is a bivalent naphthalene sulfonate with aniline moieties. Bis-ANS modulates the liquid-liquid phase separation of TDP-43 low complexity domain (LCD)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

672.85

C₃₂H₂₂K₂N₂O₆S₂

65664-81-5

Solid

Light yellow to yellow

O=S(C1=CC=CC2=C(C3=C4C=CC=C(S(=O)(O[K])=O)C4=C(NC5=CC=CC=C5)C=C3)C=CC(NC6=CC=CC=C6)=C12)(O[K])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. P Horowitz, et al. Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. J Biol Chem. 1984 Dec 10;259(23):14647-50.  [Content Brief]

  [2]. W. Michael Babinchak, et al. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Nature Communications volume 11, Article number: 5574 (2020)