BMPO (Synonyms: BocMPO)

Name: BMPO
Brand: MedChemExpress
SKU: HY-121137
Rating: 4.5 (1 reviews)

Cat. No.: HY-121137 Purity: 98.95%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BMPO (BocMPO) is a cell-permeable superior spin trap with favorable chemical and spectroscopic features. BMPO (BocMPO) can be used for detecting thiyl radicals, hydroxyl radicals, superoxide anions and glutathiyl radicals.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

BMPO 화학구조

CAS No. : 387334-31-8

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE BMPO

•ACS Appl Mater Interfaces. 2025 Jun 11;17(23):33465-33473. [Abstract]

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

BMPO is able to trap both OH and GS to form more persistent BMPO/ OH and BMPO/ SG adducts. In addition, the electron spin resonance (ESR) spectra of BMPO-derived adducts exhibit high signal-to-noise ratios in biological systems. For example, the ESR spectrum of the BMPO glutathionyl adduct (BMPO/ SG) does not fully overlap with the spectrum of its hydroxyl adduct. The favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals.^[1]
The potential toxic effects of the spin trap BMPO is measured by two estimates of cell viability (trypan blue exclusion and colony formation).^[3]
BMPO has no significant effect on cell viability at 2.5 or 25 mM in CHO cells. However, it leads to a significant increase in the number of cells that are unable to exclude Trypan blue at 50 mM BMPO. BMPO significantly reduces a 30% reduction in colony formation at 25 mM concentration in CHO cells. And, BMPO completely inhibits the colony formation of 9L tumor cells at 50 mM.^[3]
The cell function (rate of oxygen consumption) is also measured, BMPO (25 mM) significantly reduces the oxygen consumption rates in CHO cells.^[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

199.25

화학식

C₁₀H₁₇NO₃

CAS No.

387334-31-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1(C)[N+]([O-])=CCC1)OC(C)(C)C

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (501.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0188 mL	25.0941 mL	50.1882 mL
5 mM	1.0038 mL	5.0188 mL	10.0376 mL
10 mM	0.5019 mL	2.5094 mL	5.0188 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (12.55 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 98.95%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (251 KB) HNMR (226 KB) LCMS (110 KB)

Handling Instructions (2659 KB)

References

[1]. H Zhao, et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic Biol Med [Content Brief]

[2]. Hongtao Zhao, et al. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A [Content Brief]

[3]. Nadeem Khan, et al. Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med. 2003 Jun 1;34(11):1473-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.0188 mL	25.0941 mL	50.1882 mL	125.4705 mL
	5 mM	1.0038 mL	5.0188 mL	10.0376 mL	25.0941 mL
	10 mM	0.5019 mL	2.5094 mL	5.0188 mL	12.5471 mL
	15 mM	0.3346 mL	1.6729 mL	3.3459 mL	8.3647 mL
	20 mM	0.2509 mL	1.2547 mL	2.5094 mL	6.2735 mL
	25 mM	0.2008 mL	1.0038 mL	2.0075 mL	5.0188 mL
	30 mM	0.1673 mL	0.8365 mL	1.6729 mL	4.1824 mL
	40 mM	0.1255 mL	0.6274 mL	1.2547 mL	3.1368 mL
	50 mM	0.1004 mL	0.5019 mL	1.0038 mL	2.5094 mL
	60 mM	0.0836 mL	0.4182 mL	0.8365 mL	2.0912 mL
	80 mM	0.0627 mL	0.3137 mL	0.6274 mL	1.5684 mL
	100 mM	0.0502 mL	0.2509 mL	0.5019 mL	1.2547 mL

BMPO Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BMPO387334-31-8BocMPOReactive Oxygen Species (ROS)cyclic nitrone spin trapthiyl radicalshydroxyl radicalssuperoxide anionsInhibitorinhibitorinhibit

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BMPO (Synonyms: BocMPO)

고객리뷰

BMPO Related Antibodies

1 Publications Citing Use of MCE BMPO

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

BMPO Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

BMPO Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report