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BZiPAR 

Cat. No.: HY-D1685
Handling Instructions

BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease.

For research use only. We do not sell to patients.

BZiPAR Chemical Structure

BZiPAR Chemical Structure

CAS No. : 254451-46-2

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Description

BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Confocal Imaging of Trypsinogen Activation:
1. Incubate the cells according to your normal protocol.
2. Isolate clusters of acinar cells.
3. Incubate acini in extracellular solution containing 100 μM BZiPAR.
4. BZiPAR were excited with a 488-nm laser line; emission was collected in the 508- to 530-nm band for cleaved BZiPAR.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1404.44

Formula

C70H88Cl2N14O13

CAS No.
SMILES

CC[[email protected]@H](C)[[email protected]](NC(OCC1=CC=CC=C1)=O)C(N2[[email protected]@H](CCC2)C(N[[email protected]](C(NC3=CC=C(C(C(C=CC=C4)=C4C5=O)(O5)C6=C7C=C(NC([[email protected]@H](NC([[email protected]@H]8CCCN8C([[email protected]@H](NC(OCC9=CC=CC=C9)=O)[[email protected]](C)CC)=O)=O)CCCNC(N)=N)=O)C=C6)C(O7)=C3)=O)CCCNC(N)=N)=O)=O.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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BZiPAR
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HY-D1685
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