Coumarin 6 

Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λ_exc=450 nm, λ_em=505 nm)^[1][2][3].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Coumarin 6 labeled HPMC nanoparticles^[3]
1. Dissolve Coumarin 6 in DMF to prepare a 5 mg/mL organic phase solution.
2. Dissolve hydroxypropyl methyl cellulose (HPMC) in HBSS to prepare a 50 μg/mL aqueous solution.
3. Add the organic solution to the aqueous solution using a MasterFlex L/S pump (volume ratio of organic solution to aqueous solution is 1:100) with an injection rate of 36 mL/min.
4. Prepare 70 nm nanoparticles by stirring at room temperature for 5 min at a stirring speed of 9 mL/min.
5. Add 2 μg/mL nanoparticles to the MDCKII cell culture dish and incubate in the dark for 5 min, 15 min, 30 min and 60 min.
6. Add cold PBS buffer to stop the uptake of nanoparticles by MDCKII cells and clean with HBSS.
7. Use a fluorescence microscope to observe the uptake of nanoparticles by MDCKII cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

350.44

C₂₀H₁₈N₂O₂S

38215-36-0

Solid

Yellow to orange

505

450

O=C1C(C2=NC3=CC=CC=C3S2)=CC4=CC=C(N(CC)CC)C=C4O1

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Purr E, et al. Preparation of PLGA Nanoparticles Encapsulated with Fluorescent Probe Coumarin-6[J]. bioRxiv, 2019: 614875.

  [2]. Rivolta I, et al. Cellular uptake of coumarin-6 as a model drug loaded in solid lipid nanoparticles. J Physiol Pharmacol. 2011 Feb;62(1):45-53.  [Content Brief]

  [3]. Miao X, et al. Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish. ACS Appl Mater Interfaces. 2016 May 25;8(20):12620-30.  [Content Brief]