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  2. Cyanine5 DBCO

Cyanine5 DBCO (Synonyms: DBCO-Cy5; Cyanine5 dibenzocyclooctyne)

Cat. No.: HY-D1583
Handling Instructions

Cyanine5 DBCO (DBCO-Cy5) is a water-soluble, low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells). Cyanine5 DBCO can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm).

For research use only. We do not sell to patients.

Cyanine5 DBCO Chemical Structure

Cyanine5 DBCO Chemical Structure

CAS No. : 2360411-64-7

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Description

Cyanine5 DBCO (DBCO-Cy5) is a water-soluble, low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells). Cyanine5 DBCO can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell labeling (A549 cells for example)[1][2]:
1. Seed cells onto 35-mm glass-bottom dishes at a density of 3×104 (varies by cell) in 2 mL of growth media.
2. Add Ac4ManNAz (50 µM, final concentration), incubate for 3 days (generate azide groups on the surface of cells).
3. Wash cells twice with DPBS (pH 7.4).
4. Incubate cells with Cyanine5 DBCO (20 µM, final concentration) for 1 h at 37℃.
5. Rinse cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combined fixative for 15 min at 25℃.
6. Wash cells twice with DPBS (pH 7.4) and stain cells with DAPI to label nuclei.
7. Measure Cyanine5 DBCO fluorescence (Ex=635 nm, Em=650-700 nm) using a confocal laser scanning microscope.
Note:
1) Artificially introduced azide groups on the cell surface can be targeted by Cyanine5 DBCO for more than 3 days after treatment with Ac4ManNAz.
2) Degree of Cyanine5 DBCO labeling is in a dose-dependent manner.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell tracking in vivo[1][2]:
1. Seed cells onto 35-mm glass-bottom dishes at a density of 3×104 (varies by cell) in 2 mL of growth media.
2. Add Ac4ManNAz (50 µM, final concentration), incubate for 3 days (generate azide groups on the surface of cells).
3. Wash cells twice with DPBS (pH 7.4).
4. Anesthesia for mice, then inject cells (after Ac4ManNAz treatment) into the left lobe of the liver.
5. Inject Cyanine5 DBCO (25 µM, 200 µL) to tail vein (3 days post injection of cells into the liver).
6. Measure Cyanine5 DBCO fluorescence (Ex=635 nm, Em=650-700 nm). In vivo near-infrared fluorescence (NIRF) images is obtained with the in vivo imaging system.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

929.03

Formula

C53H59F6N4O2P

CAS No.
SMILES

O=C(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C\2(C)C)C=CC=C3)C(C)(C)C4=C1C=CC=C4)NCCCCCC(N5C6=C(C#CC7=C(C5)C=CC=C7)C=CC=C6)=O.[F-][P+5]([F-])([F-])([F-])([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Cyanine5 DBCO
Cat. No.:
HY-D1583
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