2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. DFHBI-1T

DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.

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CAS 番号 : 1539318-36-9

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 132 在庫あり
Solution
10 mM * 1 mL in DMSO USD 132 在庫あり
Solid
5 mg $120 在庫あり
10 mg $200 在庫あり
25 mg $420 在庫あり
50 mg $650 在庫あり
100 mg $980 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of DFHBI-1T:

DFHBI-1T 関連抗体

Top Publications Citing Use of Products

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Talanta. 2023 May 15:257:124373.

    DFHBI-1T (12.5 mM; 3 min). The feasibility of the SDAHQ platform.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816.  [Abstract]

    Normalized fluorescence intensity of a single 20 μM fluorophore (DFHBI or DFHBI-1T) and in complex with Spinach, Broccoli, and dBroccoli in HeLa cells. Count the median of different group flow cytometry green fluorescence intensity data and use dBroccoli/DFHBI-1T as 1 to calculate the normalized fluorescence intensity. Error bars indicate standard deviations (n = 3).

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2022 May 29;1209:339816.  [Abstract]

    Confocal images of control (untreated), only DFHBI-1T (20 μM), dBroccoli/DFHBI-1T, and UTRsSOD2-dBroccoli/DFHBI-1T in HeLa cells.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525.  [Abstract]

    DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. The fluorescence imaging of HeLa cell after 24 h transfection. Green signals: Broccoli, Red signals: mCherry.

    DFHBI-1T purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2021 Sep 30;41(9):BSR20211525.  [Abstract]

    DFHBI-1T could be bonded and activated by Broccoli to produce green fluorescence. DFHBI-1T was treated at 20 μM for 10 min at room temperature. Flow cytometry histograms showing the green fluorescence intensity of Broccoli induced by different promoters.

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    • 参考文献

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    製品説明

    DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells[1][2].

    体外実験

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
    1. Preparation of stock solution:
    Use anhydrous DMSO to prepare a 50 mM DFHBI-1T stock solution.
    2. Preparation of working solution:
    Dilute the stock solution with serum-free medium to prepare a 20 μM DFHBI-1T working solution.
    Note: Please adjust the concentration of DFHBI-1T working solution according to actual conditions and prepare it before use.
    3. Specific staining steps:
    1) Inoculate cells expressing Spinach2-tagged RNA in a suitable culture dish or culture plate and culture under standard cell culture conditions. 2) Aspirate the old medium and gently wash the cells twice with pre-warmed PBS to remove residual serum and cell debris.
    3) Add 20 μM DFHBI-1T working solution to the cells, ensuring complete coverage of the cell monolayer.
    4) Incubate the cells with the dye for 10 min in a 37°C, 5% CO2 humidified incubator.
    5) After incubation, aspirate the dye-containing medium and wash the cells three times with pre-warmed PBS to remove unbound DFHBI-1T.
    6) Replace PBS with fresh cell culture medium or imaging buffer.
    7) Immediately image the cells using a fluorescence microscope equipped with a GFP filter set to detect fluorescence of the Spinach2-DFHBI-1T complex.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DFHBI-1T 関連抗体
    分子量

    320.21

    分子式

    C13H9F5N2O2
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1N(CC(F)(F)F)C(C)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1230 mL 15.6148 mL 31.2295 mL
    5 mM 0.6246 mL 3.1230 mL 6.2459 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション
    Dyeing Example
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1230 mL 15.6148 mL 31.2295 mL 78.0738 mL
    5 mM 0.6246 mL 3.1230 mL 6.2459 mL 15.6148 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL 7.8074 mL
    15 mM 0.2082 mL 1.0410 mL 2.0820 mL 5.2049 mL
    20 mM 0.1561 mL 0.7807 mL 1.5615 mL 3.9037 mL
    25 mM 0.1249 mL 0.6246 mL 1.2492 mL 3.1230 mL
    30 mM 0.1041 mL 0.5205 mL 1.0410 mL 2.6025 mL
    40 mM 0.0781 mL 0.3904 mL 0.7807 mL 1.9518 mL
    50 mM 0.0625 mL 0.3123 mL 0.6246 mL 1.5615 mL
    60 mM 0.0520 mL 0.2602 mL 0.5205 mL 1.3012 mL
    80 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9759 mL
    100 mM 0.0312 mL 0.1561 mL 0.3123 mL 0.7807 mL
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    DFHBI-1T Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DFHBI-1T1539318-36-9DNA StainRNAaptamersprobeSpinachSpinach2iSpinachBroccolilivecellsInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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