DiBAC4(3) (solution) 

DiBAC4 (3) (solution) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm). DiBAC4 (3) can be used to measure cell membrane potential.
Solvent and concentration: DMSO: 10 mM

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Solution Preparation
Dilute to 10-40 μM using Hanks and 20 mM Hepes buffer (HHBS) or other buffers (such as pH 7 and containing 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
Note: The working solution should be prepared and used immediately. Store in a dark place.

2. Cell Staining 2.1 Place the cells in a transparent black 96-well microplate at the bottom.
2.2 Add 100 μL of DiBAC4(3) to the cell plate.
2.3 Add the dye and the template to the cell culture box and incubate for 30 to 60 minutes.
2.4 The membrane potential was determined by monitoring the fluorescence intensity at 490/505 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

516.64

C₂₇H₄₀N₄O₆

70363-83-6

Liquid (Density: 1.201±0.06 g/cm³)

Yellow to orange

O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light. Avoid repeated freezing and thawing

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light. Avoid repeated freezing and thawing)

• [1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.  [Content Brief]