Trypan red  (Synonyms: C.I. 22850)

Trypan red is a vital stain.

1002.80

C₃₂H₁₉N₆Na₅O₁₅S₅

574-64-1

Solid

Brown to black

584

544

O=S(C1=CC(C2=CC=C(/N=N/C3=C4C=CC(S(=O)([O-])=O)=CC4=CC(S(=O)([O-])=O)=C3N)C=C2)=CC=C1/N=N/C5=C6C=CC(S(=O)([O-])=O)=CC6=CC(S(=O)([O-])=O)=C5N)([O-])=O.[Na+].[Na+].[Na+].[Na+].[Na+]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. POSCHEL PH. An effect of the vital dye trypan red on approach-avoidance conflict. J Comp Physiol Psychol. 1958 Aug;51(4):448-51.  [Content Brief]

  [2]. HURST EW, et al. The prevention of encephalitis due to the viruses of eastern equine encephalomyelitis and louping-ill: experiments with trypan red, mepacrine, and many other substances. Br J Pharmacol Chemother. 1952 Sep;7(3):455-72.  [Content Brief]