1. Dye Reagents
  2. DiBAC4(3)

DiBAC4(3) 

Cat. No.: HY-101892 Purity: ≥98.0%
Handling Instructions

DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).

For research use only. We do not sell to patients.

DiBAC4(3) Chemical Structure

DiBAC4(3) Chemical Structure

CAS No. : 70363-83-6

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 488 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 488 In-stock
Estimated Time of Arrival: December 31
Solid
25 mg USD 444 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE DiBAC4(3)

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).

In Vitro

The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

516.63

Formula

C27H40N4O6

CAS No.
SMILES

O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 60 mg/mL (116.14 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9356 mL 9.6781 mL 19.3562 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL
10 mM 0.1936 mL 0.9678 mL 1.9356 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Product Name:
DiBAC4(3)
Cat. No.:
HY-101892
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