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  2. Fluorescent Dye
  3. DiBAC4(3)


Cat. No.: HY-101892 Purity: ≥98.0%
COA Handling Instructions

DiBAC4(3) is a voltage-sensitive fluorescent dyeex=490 nm, λem=505 nm).

For research use only. We do not sell to patients.

DiBAC4(3) Chemical Structure

DiBAC4(3) Chemical Structure

CAS No. : 70363-83-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 80 In-stock
10 mM * 1 mL in DMSO USD 80 In-stock
5 mg USD 70 In-stock
10 mg USD 105 In-stock
25 mg USD 210 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review


DiBAC4(3) is a voltage-sensitive fluorescent dyeex=490 nm, λem=505 nm).

In Vitro

The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







Pink to red

Emission (Em)


Excitation (Ex)





Room temperature in continental US; may vary elsewhere.


4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (96.78 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9356 mL 9.6781 mL 19.3562 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL
10 mM 0.1936 mL 0.9678 mL 1.9356 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: ≥98.0%

Cell Assay

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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DiBAC4(3) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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