1. Others
  2. Fluorescent Dye
  3. DiBAC4(3)

DiBAC4(3) is a voltage-sensitive fluorescent dyeex=490 nm, λem=505 nm).

For research use only. We do not sell to patients.

DiBAC4(3) Chemical Structure

DiBAC4(3) Chemical Structure

CAS No. : 70363-83-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 80 In-stock
Solution
10 mM * 1 mL in DMSO USD 80 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 105 In-stock
25 mg USD 210 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

DiBAC4(3) is a voltage-sensitive fluorescent dyeex=490 nm, λem=505 nm).

In Vitro

The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

516.63

Formula

C27H40N4O6

CAS No.
Appearance

Solid

Color

Pink to red

Emission (Em)

505

Excitation (Ex)

490

SMILES

O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (96.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9356 mL 9.6781 mL 19.3562 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

References
Cell Assay
[1]

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9356 mL 9.6781 mL 19.3562 mL 48.3905 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL 9.6781 mL
10 mM 0.1936 mL 0.9678 mL 1.9356 mL 4.8391 mL
15 mM 0.1290 mL 0.6452 mL 1.2904 mL 3.2260 mL
20 mM 0.0968 mL 0.4839 mL 0.9678 mL 2.4195 mL
25 mM 0.0774 mL 0.3871 mL 0.7742 mL 1.9356 mL
30 mM 0.0645 mL 0.3226 mL 0.6452 mL 1.6130 mL
40 mM 0.0484 mL 0.2420 mL 0.4839 mL 1.2098 mL
50 mM 0.0387 mL 0.1936 mL 0.3871 mL 0.9678 mL
60 mM 0.0323 mL 0.1613 mL 0.3226 mL 0.8065 mL
80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6049 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

DiBAC4(3) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DiBAC4(3)
Cat. No.:
HY-101892
Quantity:
MCE Japan Authorized Agent: