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  4. DiBAC4(3)

DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). DiBAC4(3) can be used to measure cell membrane potential.

For research use only. We do not sell to patients.

CAS No. : 70363-83-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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Customer Review

Based on 15 publication(s) in Google Scholar

Other Forms of DiBAC4(3):

DiBAC4(3) Related Antibodies

Top Publications Citing Use of Products

    DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 25:57:646-659.  [Abstract]

    DiBAC4(3) (300 nM, 30 min) was used for membrane potential measurement in Caco-2 cells.

    DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Food Res Int. 2025 Dec;221(Pt 4):117517.

    DiBAC4(3) at a final concentration of 2.5 μg/mL for 15 min at 25 °C protected from light. was used for membrane potential measurement in L. monocytogenes EGD-e.

    DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Aug 28:179:117352.  [Abstract]

    DiBAC4(3) at a final concentration of 1 μM for 10 min was used for membrane potential measurement in C. albicans SC5314 cells.

    DiBAC4(3) purchased from MedChemExpress. Usage Cited in: J Cancer Res Clin Oncol. 2023 Feb;149(2):683-699.  [Abstract]

    DiBAC4(3) at a final concentration of 100 nM for 20 min was used for membrane potential measurement in T24 cells.

    DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2020 Nov.

    DiBAC4(3) at a final concentration of 100 nM for 20 min at room temperature was used for membrane potential measurement in T24 cells.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). DiBAC4(3) can be used to measure cell membrane potential.

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 10-40 mM.
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 10-40 μM with Hanks and 20 mM Hepes buffer (HHBS) or other buffer, such as pH 7 with 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 Cells are plated in clear-bottomed black 96-well microplates.
    2.2 Add 100 μL DiBAC4(3) into the cell plate.
    2.3 Incubate the dye-loading plate in a cell incubator for 30 to 60 minutes.
    2.4 Run the membrane potential assay by monitoring the fluorescence intensity at Ex/Em = 490/505 nm.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DiBAC4(3) Related Antibodies
    Molecular Weight

    516.63

    Formula

    C27H40N4O6
    CAS No.
    Appearance

    Solid

    Color

    Pink to red

    Emission (Em)

    505
    Excitation (Ex)

    490
    SMILES

    O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (96.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9356 mL 9.6781 mL 19.3562 mL
    5 mM 0.3871 mL 1.9356 mL 3.8712 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.91%

    SDS (252 KB)

    COA (247 KB) HNMR (253 KB) RP-HPLC (253 KB) LCMS (88 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9356 mL 9.6781 mL 19.3562 mL 48.3905 mL
    5 mM 0.3871 mL 1.9356 mL 3.8712 mL 9.6781 mL
    10 mM 0.1936 mL 0.9678 mL 1.9356 mL 4.8391 mL
    15 mM 0.1290 mL 0.6452 mL 1.2904 mL 3.2260 mL
    20 mM 0.0968 mL 0.4839 mL 0.9678 mL 2.4195 mL
    25 mM 0.0774 mL 0.3871 mL 0.7742 mL 1.9356 mL
    30 mM 0.0645 mL 0.3226 mL 0.6452 mL 1.6130 mL
    40 mM 0.0484 mL 0.2420 mL 0.4839 mL 1.2098 mL
    50 mM 0.0387 mL 0.1936 mL 0.3871 mL 0.9678 mL
    60 mM 0.0323 mL 0.1613 mL 0.3226 mL 0.8065 mL
    80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6049 mL
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    DiBAC4(3) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DiBAC4(3)70363-83-6Fluorescent DyeInhibitorinhibitorinhibit

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