DiBAC4(3) (solution)
Based on 1 Customer Validation
DiBAC4 (3) (solution) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). DiBAC4 (3) can be used to measure cell membrane potential.
Solvent and concentration: DMSO: 10 mM
For research use only. We do not sell to patients.
- CAS No.: 70363-83-6
- Formula: C27H40N4O6
- Molecular Weight:516.64
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Solvent and concentration: DMSO: 10 mM
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Solution Preparation
Dilute to 10-40 μM using Hanks and 20 mM Hepes buffer (HHBS) or other buffers (such as pH 7 and containing 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
Note: The working solution should be prepared and used immediately. Store in a dark place.
2. Cell Staining
2.1 Place the cells in a transparent black 96-well microplate at the bottom.
2.2 Add 100 μL of DiBAC4(3) to the cell plate.
2.3 Add the dye and the template to the cell culture box and incubate for 30 to 60 minutes.
2.4 The membrane potential was determined by monitoring the fluorescence intensity at 490/505 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 70363-83-6
-
Appearance Liquid (Density: 1.201±0.06 g/cm3)
-
Molecular Weight 516.64
-
Formula C27H40N4O6
-
Color Yellow to orange
-
SMILES
O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (270 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DiBAC4(3) (solution) Related Classifications
HY-DY1039 Related Classifications
Powered by Bioz