2. Metabolic Enzyme/Protease
  3. FXR
  4. DY268

DY268 is a farnesoid X receptor (FXR) antagonist (IC50=7.5 nM). It inhibits FXR transactivation in a cell-based assay with an IC50 value of 468 nM. DY268 can be used in the study of drug-induced liver injury (DILI).

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CAS 番号 : 1609564-75-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 123 在庫あり
Solution
10 mM * 1 mL in DMSO USD 123 在庫あり
Solid
5 mg $100 在庫あり
10 mg $160 在庫あり
25 mg $320 在庫あり
50 mg $580 在庫あり
100 mg $950 在庫あり
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カスタマーレビュー

Based on 3 publication(s) in Google Scholar

DY268 関連抗体

Top Publications Citing Use of Products
2D/3D Cell Culture and Differentiation
Cell Proliferation/Viability Assay
RT-PCR
WB

    DY268 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 May 9:115675.  [Abstract]

    Schematic of FXR inhibition in iHOs. Representative bright-field images of iHOs (left) and iHOs supplemented with two different FXR inhibitors (middle: DY268 (10 μm), right: ZGG). Red arrows indicate grape-like organoids.

    DY268 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 May 9:115675.  [Abstract]

    Counts of grape-like and total organoid formation at passage 0 (n = 5; P11025: 3, ChiPSC18: 2) based on bright-field images. Organoids with a long diameter greater than 200 mm were counted. The number of grape-like organoids was significantly lower in the FXR-inhibitor DY268 (10 μm)-supplemented groups.

    DY268 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 May 9:115675.  [Abstract]

    Expression levels of representative NR1H4 target genes (NR0B2, FGF19, and ABCB11) are shown on top. NR0B2 expression was significantly lower in both FXR-inhibitor DY268 (10 μm)-supplemented groups. Hepatocyte marker gene expression was also significantly lower in the FXR-inhibitor-supplemented groups (bottom).

    DY268 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2026 Jan:210:108187.  [Abstract]

    Effects of SBI-115 and DY268 on IPEC-J2 cells. Compounds at concentrations of 0 nM, 100 nM, 1 μM, 10 μM, and 50 μM were added to IPEC-J2 cells, which were then cultured for 48 h. Cell viability was evaluated using the CCK8 assay.

    DY268 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2026 Jan:210:108187.  [Abstract]

    IPEC-J2 cells were pretreated with LCA and inhibitor (DY268; 100 nM). PEDV N mRNA levels were then analyzed through qPCR.

    DY268 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2026 Jan:210:108187.  [Abstract]

    IPEC-J2 cells were pretreated with LCA and SBI-115 or DY268 (100 nM). PEDV N protein levels were analyzed through Western blotting.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DY268 is a farnesoid X receptor (FXR) antagonist (IC50=7.5 nM). It inhibits FXR transactivation in a cell-based assay with an IC50 value of 468 nM. DY268 can be used in the study of drug-induced liver injury (DILI)[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    HEK-293T IC50
    468.5 nM
    Compound: 4j, DY268
    Inhibition of GW4064-induced transactivation of FXR (unknown origin) expressed in HEK293T cells after 16 hrs by beta-lactamase reporter gene assay
    Inhibition of GW4064-induced transactivation of FXR (unknown origin) expressed in HEK293T cells after 16 hrs by beta-lactamase reporter gene assay
    		[PMID: 24775917]
    体外実験

    DY268 (10 μM) treatment from 13 dpf to 15 dpf increased Bhmt expression in 15-dpf Tg(fabp10a:pt-β-catenin) livers compared with DMSO treatment[2].
    DY268 exhibits a>25% drop in ATP relative to vehicle-treated control at the highest concentration tested[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DY268 関連抗体
    分子量

    560.66

    分子式

    C30H32N4O5S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=CN(CC2=CC(OC)=CC=C2)N=C1C3=CC=C(C)C=C3)NC4=CC=C(C)C(S(=O)(N5CCOCC5)=O)=C4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (89.18 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7836 mL 8.9181 mL 17.8361 mL
    5 mM 0.3567 mL 1.7836 mL 3.5672 mL
    10 mM 0.1784 mL 0.8918 mL 1.7836 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (8.92 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (8.92 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.48%

    COA (251 KB) HNMR (203 KB) RP-HPLC (224 KB) MS (146 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7836 mL 8.9181 mL 17.8361 mL 44.5903 mL
    5 mM 0.3567 mL 1.7836 mL 3.5672 mL 8.9181 mL
    10 mM 0.1784 mL 0.8918 mL 1.7836 mL 4.4590 mL
    15 mM 0.1189 mL 0.5945 mL 1.1891 mL 2.9727 mL
    20 mM 0.0892 mL 0.4459 mL 0.8918 mL 2.2295 mL
    25 mM 0.0713 mL 0.3567 mL 0.7134 mL 1.7836 mL
    30 mM 0.0595 mL 0.2973 mL 0.5945 mL 1.4863 mL
    40 mM 0.0446 mL 0.2230 mL 0.4459 mL 1.1148 mL
    50 mM 0.0357 mL 0.1784 mL 0.3567 mL 0.8918 mL
    60 mM 0.0297 mL 0.1486 mL 0.2973 mL 0.7432 mL
    80 mM 0.0223 mL 0.1115 mL 0.2230 mL 0.5574 mL
    • No file chosen (Maximum size is: 1024 Kb)
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    DY268 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DY2681609564-75-1DY 268DY-268FXRNR1H4Inhibitorinhibitorinhibit

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    製品名:
    DY268
    製品番号:
    HY-110267
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