2. Metabolic Enzyme/Protease
  3. Fat Mass and Obesity-associated Protein (FTO)
  4. FB23

FB23 is a potent and selective FTO demethylase inhibitor with an IC50 of 60 nM. FB23 directly binds to FTO and selectively inhibits FTO's mRNA N6-methyladenosine (m6A) demethylase activity.

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FB23

FB23 Chemical Structure

CAS No. : 2243736-35-6

Size Prix Stock Quantité
Échantillon gratuit (0.1 - 0.2 mg)   Appliquer maintenant  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
25 mg En stock
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Based on 2 publication(s) in Google Scholar

FB23 Related Antibodies

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  • Activité biologique

  • Pureté et documentation

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Description

FB23 is a potent and selective FTO demethylase inhibitor with an IC50 of 60 nM. FB23 directly binds to FTO and selectively inhibits FTO's mRNA N6-methyladenosine (m6A) demethylase activity[1].

IC50 & Target

IC50: 60 nM (FTO)[1]
Cellular Effect
Cell Line Type Value Description References
NB-4 IC50
44.8 3
Compound: FB23
Antiproliferative activity against human NB4 cells measured after 72 hrs by celltiter96 aqueous non-radioactive cell proliferation assay
Antiproliferative activity against human NB4 cells measured after 72 hrs by celltiter96 aqueous non-radioactive cell proliferation assay
[PMID: 37974948]
NB-4 IC50
44.8 3
Compound: FB23
Antiproliferative activity against human NB4 cells measured after 72 hrs by celltiter96 aqueous non-radioactive cell proliferation assay
Antiproliferative activity against human NB4 cells measured after 72 hrs by celltiter96 aqueous non-radioactive cell proliferation assay
[PMID: 37974948]
In Vitro

FB23 (72 hours) treatment inhibits acute myeloid leukemia (AML) cells proliferation with IC50 values of 44.8 μM, 23.6 μM for NB4 and MONOMAC6 AML cells[1].
FB23 treatment causes the significant suppression of MYC targets, E2F targets, and G2M checkpoint signal cascades, which may contribute to the inhibitory effects of FTO inhibitors and FTO KD on cell cycle and proliferation. FB23 treatments activates apoptosis and p53 pathways[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FB23 Related Antibodies
In Vivo

A single dose of 3 mg/kg FB23 is i.p. administrated to Sprague Dawley (SD) rats for the pharmacokinetic profile. The Cmax and Tmax value of FB23 are 142.5 ng/mL and 0.4 hr, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Masse moléculaire

377.22

Formule

C18H14Cl2N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=CC=CC=C1NC2=C(Cl)C=C(C3=C(C)ON=C3C)C=C2Cl
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : 125 mg/mL (331.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6510 mL 13.2549 mL 26.5097 mL
5 mM 0.5302 mL 2.6510 mL 5.3019 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureté et documentation
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6510 mL 13.2549 mL 26.5097 mL 66.2743 mL
5 mM 0.5302 mL 2.6510 mL 5.3019 mL 13.2549 mL
10 mM 0.2651 mL 1.3255 mL 2.6510 mL 6.6274 mL
15 mM 0.1767 mL 0.8837 mL 1.7673 mL 4.4183 mL
20 mM 0.1325 mL 0.6627 mL 1.3255 mL 3.3137 mL
25 mM 0.1060 mL 0.5302 mL 1.0604 mL 2.6510 mL
30 mM 0.0884 mL 0.4418 mL 0.8837 mL 2.2091 mL
40 mM 0.0663 mL 0.3314 mL 0.6627 mL 1.6569 mL
50 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3255 mL
60 mM 0.0442 mL 0.2209 mL 0.4418 mL 1.1046 mL
80 mM 0.0331 mL 0.1657 mL 0.3314 mL 0.8284 mL
100 mM 0.0265 mL 0.1325 mL 0.2651 mL 0.6627 mL
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FB23 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FB232243736-35-6FB 23FB-23Fat Mass and Obesity-associated Protein (FTO)FTON6-methyladenosinedemethylaseleukemogenesisanti-proliferativeInhibitorinhibitorinhibit

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Cat. No.:
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