1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. GAPDH, rabbit muscle

GAPDH, rabbit muscle  (Synonyms: GADPH; G3PDH; Glyceraldehyde phosphate dehydrogenase)

Cat. No.: HY-P2804
Instrucciones de manejo Technical Support

Glyceraldehyde phosphate dehydrogenase (EC 1.2.1.12) is the target of anti-thymocyte and anti-apoptotic agents. Glyceraldehyde phosphate dehydrogenase catalyzes the chain oxidation of reduced nicotinamide adenine dinucleotide by perhydroxyl radicals.

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GAPDH, rabbit muscle

GAPDH, rabbit muscle Estructura química

No. CAS : 9001-50-7

Tamaño Precio Stock Cantidad
100 U En stock
500 U En stock

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Based on 1 publication(s) in Google Scholar

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Descripciòn

Glyceraldehyde phosphate dehydrogenase (EC 1.2.1.12) is the target of anti-thymocyte and anti-apoptotic agents. Glyceraldehyde phosphate dehydrogenase catalyzes the chain oxidation of reduced nicotinamide adenine dinucleotide by perhydroxyl radicals[1][2].

In Vitro

Product Information
This product is recombinantly produced and can be used for enzymatic determination of glycerol and triglycerides after combining with glycerol kinase.

Molecular weight: about 73,600; subunit molecular weight: about 36,800.
pH stability: 6.5 - 10.0, optimum pH 9.0
Km: (90 mM diethylene glycol buffer pH 9.0, 37°C) Glycerol-3-phosphate: 0.119 mM; NAD+: 0.036 mM.

Instructions
Dissolved in ddH2O (25 mg/mL), avoid repeated freezing and thawing, store at 2-8°C and use within 1 week. Or dissolve in buffer according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

No. CAS
Appearance

Solid

Color

White to light yellow

SMILES

[GAPDH, rabbit muscle]

EC Number

1.2.1.12

Specific Activity

≥75 U/mg protein

Unit Definition

One unit is defined as the amount of enzyme that reduce 1.0 μmole of 3-phosphoglycerate to Dglyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25°C.

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvente y solubilidad
In Vitro: 

H2O : ≥ 25 mg/mL

*"≥" means soluble, but saturation unknown.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
GAPDH, rabbit muscle
Cat. No.:
HY-P2804
Cantidad:
MCE Japan Authorized Agent: