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  4. HKOCl-3

HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid.Ex: 490 nm; Em 527 nm.

For research use only. We do not sell to patients.

HKOCl-3

HKOCl-3 Chemical Structure

CAS No. : 2031170-80-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 2 publication(s) in Google Scholar

HKOCl-3 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid.Ex: 490 nm; Em 527 nm[1].

IC50 & Target

Ex: 490 nm; Em 527 nm
In Vitro

HKOCl-3 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-3 (10 μM) shows a much higher increase in fluorescence intensity of compound HKOCl-3 than treatment with other ROS (10 μM) and RNS (100 μM) [1].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-2 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 1-2 μM with with PBS or serum-free medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are washed with PBS
2.2 Add HKOCl-3 (1 or 2 μM) in 1.5-mL HBSS supplemented with 0.1% Chloramphenicol (HY-B0239) and 0.6 mM L-Arginine (HY-N0455).
2.3 Cells are typically incubated for 30 min at 37°C.
2.4 Fluorescence microscopy detection (Ex/Em = 488/500-530 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKOCl-3 Related Antibodies
Molecular Weight

493.29

Formula

C26H14Cl2O6
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

527
Excitation (Ex)

490
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(OC5=CC(Cl)=C(O)C(Cl)=C5)C=C3)C6=C1C=CC=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (202.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0272 mL 10.1360 mL 20.2721 mL
5 mM 0.4054 mL 2.0272 mL 4.0544 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.51%

SDS (252 KB)

COA (252 KB) LCMS (108 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0272 mL 10.1360 mL 20.2721 mL 50.6801 mL
5 mM 0.4054 mL 2.0272 mL 4.0544 mL 10.1360 mL
10 mM 0.2027 mL 1.0136 mL 2.0272 mL 5.0680 mL
15 mM 0.1351 mL 0.6757 mL 1.3515 mL 3.3787 mL
20 mM 0.1014 mL 0.5068 mL 1.0136 mL 2.5340 mL
25 mM 0.0811 mL 0.4054 mL 0.8109 mL 2.0272 mL
30 mM 0.0676 mL 0.3379 mL 0.6757 mL 1.6893 mL
40 mM 0.0507 mL 0.2534 mL 0.5068 mL 1.2670 mL
50 mM 0.0405 mL 0.2027 mL 0.4054 mL 1.0136 mL
60 mM 0.0338 mL 0.1689 mL 0.3379 mL 0.8447 mL
80 mM 0.0253 mL 0.1267 mL 0.2534 mL 0.6335 mL
100 mM 0.0203 mL 0.1014 mL 0.2027 mL 0.5068 mL
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HKOCl-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKOCl-32031170-80-4HKOCl3HKOCl 3Fluorescent Dyefluorogenicmitochondriahypochlorous acidhydroxyl radicalInhibitorinhibitorinhibit

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