Cat. No.: HY-N0455

L-Arginine

23 Cited Publications

Customer Review

Based on 23 publication(s) in Google Scholar

L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis.

For research use only. We do not sell to patients.

Purity: 99.89%
CAS No.: 74-79-3
Formula: C₆H₁₄N₄O₂
Molecular Weight:174.20

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

L-Arginine Chemical Structure

CAS No.: 74-79-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Induced Disease Models
Chemical Information
Publications (23)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

eNOS

Arg

All NO Synthase Isoforms :

All Endogenous Metabolite Isoforms :

All Ligands for E3 Ligase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	283 μM Compound: 6322	Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells	10.5281/zenodo.6782708
HEK293	EC₅₀	283 μM Compound: 6322	Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863) Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)	10.5281/zenodo.6782708

In Vitro

Arginine is an α-amino acid. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that code for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual^[1].
L-Arginine is associated with a decrease in cardiac index. L-Arginine (450 mg/kg during a 15-minute period) amplifies and sustains the hyperemia (38%) and increases absolute brain blood flow after eNOS upregulation by chronic Simvastatin (HY-17502) (2 mg/kg; sc; daily for 14 days) in SV-129 mice^[2]^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

L-Arginine has been widely accepted as a method to induce experimental acute pancreatitis^[4]^[5].
Dose reference for L-Arginine induction^[4]^[5]:
(1) Model animal: Male Wistar albino rats
Acute pancreatitis: single i.p. injection of 500 mg of L-arginine/100 g body weight
(2) Model animal: Male ICR mice
Acute pancreatitis: i.p. with 2.25 g/kg body weight of L-Arginine hourly for 2 hours
Dissolution method of L-Arginine^[5]:
L-arginine solution was prepared by dissolving L-arginine powder in 0.9% normal saline and adjusting the pH to 7 with 5 N HCl.

Induction of Experimental Acute Pancreatitis^[4]^[5]

Background

L-Arginine is a substrate for endothelial nitric oxide synthase (eNOS) to produce NO, which can be metabolized into nitric oxide (NO), polyamines or L-proline, stimulating inflammatory responses. L-Arginine can also selectively destroy pancreatic acinar cells, leading to acute necrotizing pancreatitis.

Specific Modeling Methods

Rat^[4]: Wistar albino rats • Male • 150 g
Administration: 250 mg, 500 mg, 750 mg every 100 g body weight • ip • single dose • starved for 15 hours

Mice^[5]: ICT mouse • Male • 4-week-old • 25-30 g
Administration: 450 mg/100 g body weight • ip • twice dosages • sacrificed at 72 hours

Note

(1) When the dose exceeds 500 mg/100 g body weight, most rats will die within a few hours after injection; When the dose is less than 250 mg, the induced symptoms of pancreatic disease will be mild^[4].
(2) Dissolution method in Reference: L-Arginine solution was prepared by dissolving L-Arginine powder in 0.9% normal saline and adjusting pH to 7 with 5N hydrochloric acid^[5].

Modeling Indicators

Phenotypic changes: weight loss, lower activity, softer hair.

Tissue morphology: pancreatic edema, damage, atrophy, granulation tissue contraction and chalky spots (location of fat necrosis).

Cellular changes: basophils decrease and vesicles, cell pyknosis, nuclear fragmentation. Pancreatic albino cells disappear and die, and pancreatic tissue is replaced by monocytes and fibroblasts.

Molecular changes: serum amylase levels increase, and fat necrosis is obvious.

Correlated Product(s): Curcumin (HY-N0005)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 74-79-3
Appearance Solid
Molecular Weight 174.20
Formula C₆H₁₄N₄O₂
Color White to off-white
SMILES

N[C@@H](CCCNC(N)=N)C(O)=O
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (23)

All Cell Imaging/StainingIn Vivo Efficacy StudyFlow Cytometry

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Journal Impact Factor

Signal Transduct Target Ther

Arginase 1 drives mitochondrial cristae remodeling and PANoptosis in ischemia/hypoxia-induced vascular dysfunction. [Abstract]

2025 May 28;10(1):167. PMID: 40425583
Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Nat Nanotechnol

Annexin A1 mRNA-loaded liposomes alleviate acute pancreatitis by suppressing STING pathway and promoting efferocytosis in macrophages. [Abstract]

2025 Aug 11. PMID: 40789923
Nat Protoc

An unbiased method for evaluating the genome-wide specificity of base editors in rice. [Abstract]

2021 Jan;16(1):431-457. PMID: 33349703
Autophagy

ESRRA-ATG5-Mediated mitophagy enhances arginine metabolism to alleviate diabetic kidney disease. [Abstract]

2025 Dec 23:1-25. PMID: 41376268
Adv Sci (Weinh)

Targeting the SOX9/TIMP1 Axis with iRGD-Conjugated Nanoplatform Enhances Dendritic Cell Function and Photodynamic Immunotherapy in Gastric Cancer. [Abstract]

2025 Nov 21:e10500. PMID: 41270217

L-Arginine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e10500. [Abstract]

Intracellular ROS and NO generation were measured under different L-Arg (25-200 mg/mL) concentrations following iRGD NPs@si-SOX9/CL+L (24 h) treatment using DCFH-DA and DAF-FM DA probes
Cell Rep Med

Targeting AQP5-mediated arginine deprivation in gastric cancer stem cells restores NK cell anti-tumor immunity. [Abstract]

2025 Sep 16;6(9):102333. PMID: 40961922

L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333. [Abstract]

Images and tumor volume in subcutaneous tumors established with AQP5⁺ or AQP5⁻ MFC cells in C57BL/6J mice treated with daily oral gavage of different concentrations of L-Arginine (1-2 g/kg; p.o. daily for 27 d) or PBS.

L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333. [Abstract]

Proportion of NK cells in subcutaneous tumors detected by flow cytometry (AQP5⁺); AQP5⁺ + L-Arginine (1 g/kg daily oral gavage); AQP5⁺ + L-Arginine (2 g/kg daily oral gavage); AQP5^-).

L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333. [Abstract]

NK cells co-cultured with AQP5⁺ AGS cells, AQP5^- AGS cells, or AQP5⁺ AGS cells supplemented with 40 nM L-Arginine for 48 h and co-cultured with AGS cells. Images detected by live cell workstation; NK cells were labeled green by CFSE, and AGS cells were labeled red.
Int J Nanomedicine

A Multifunctional Nanodrug Co-Delivering VEGF-siRNA and Dexamethasone for Synergistic Therapy in Ocular Neovascular Diseases. [Abstract]

2024 Nov 21:19:12369-12387. PMID: 39606561
J Agric Food Chem

Protocatechuic Acid, a Gut-Derived Dietary Metabolite, Attenuates Endothelial Dysfunction via GPER-Mediated NO Signaling. [Abstract]

2026 Feb 11;74(5):4582-4598. PMID: 41605862
Nutrients

Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate. [Abstract]

2023 Oct 18;15(20):4427. PMID: 37892502

L-Arginine purchased from MedChemExpress. Usage Cited in: Nutrients. 2023 Oct 18;15(20):4427. [Abstract]

A ketogenic diet exacerbated L-Arginine (4 g/kg; hourly i.p. for 2 times )-induced acute pancreatitis. H&E staining and TEM sections of pancreatic tissue.
Int Immunopharmacol

Targeting periodontal inflammatory microenvironment to ameliorate periodontitis: A nitrogen ions implantation technique. [Abstract]

2025 Dec 24:170:116055. PMID: 41448003
Molecules

Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]

2025 Mar 9;30(6):1224. PMID: 40142001
Genes Immun

Annexin A1 regulates inflammatory-immune response and reduces pancreatic and extra- pancreatic injury during severe acute pancreatitis. [Abstract]

2025 Apr;26(2):124-136. PMID: 40025269
FASEB J

Spatial Metabolism of Primary Limited Cutaneous Amyloidosis Based on Mass Spectrometry Imaging. [Abstract]

2025 Sep 30;39(18):e71057. PMID: 40955599
J Biol Chem

Nitric oxide mediates ET-1-induced-inhibition of NPPB-sensitive Cl- currents in the early distal convoluted tubule of the mouse kidney. [Abstract]

2026 Jan 23;302(3):111202. PMID: 41581878
J Biomed Mater Res A

Hyaluronic Acid-Chitosan Nanoparticles Encapsulating Gal-9 Alleviate Severe Acute Pancreatitis by Promoting M2 Macrophage Polarization. [Abstract]

2025 Dec;113(12):e70006. PMID: 41307175
Viruses

Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1. [Abstract]

2021 Jun 26;13(7):1236. PMID: 34206713
Dig Dis Sci

Dysregulated B7H4/JAK2/STAT3 Pathway Involves in Hypertriglyceridemia Acute Pancreatitis and Is Attenuated by Baicalin. [Abstract]

2023 Feb;68(2):478-486. PMID: 35781653
Pancreas

Nuclear Factor-κB Increases Intracellular Calcium by Upregulation of Na+-Ca2+ Exchanger 1 in Cerulein-Induced Acute Pancreatitis. [Abstract]

2020 Jan;49(1):111-119. PMID: 31856086
Trop J Pharm Res

2022 Jun 4.
SSRN

2025 Dec 17.
Patent

US20230090691A1.
Patent

US20200352840A1.

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (287.03 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7405 mL

28.7026 mL

57.4053 mL

5 mM

1.1481 mL

5.7405 mL

11.4811 mL

10 mM

0.5741 mL

2.8703 mL

5.7405 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tapiero H, et al. I. Arginine. Biomed Pharmacother. 2002 Nov;56(9):439-45. [Content Brief]

[2]. Bakker J, et al. Administration of the nitric oxide synthase inhibitor NG-methyl-L-arginine hydrochloride (546C88) by intravenous infusion for up to 72 hours can promote the resolution of shock in patients with severe sepsis: results of a randomized, double-blind, placebo-controlled multicenter study (study no. 144-002). Crit Care Med. 2004 Jan;32(1):1-12. [Content Brief]

[3]. Yamada M, et al. Endothelial nitric oxide synthase-dependent cerebral blood flow augmentation by L-arginine after chronic statin treatment. J Cereb Blood Flow Metab. 2000 Apr;20(4):709-17. [Content Brief]

[4]. Mizunuma T, et al. Effects of injecting excess arginine on rat pancreas. J Nutr. 1984 Mar;114(3):467-71. [Content Brief]

[5]. Siriviriyakul P, et al. Effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis in mice. Heliyon. 2019 Aug 27;5(8):e02222. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	5.7405 mL	28.7026 mL	57.4053 mL	143.5132 mL
	5 mM	1.1481 mL	5.7405 mL	11.4811 mL	28.7026 mL
	10 mM	0.5741 mL	2.8703 mL	5.7405 mL	14.3513 mL
	15 mM	0.3827 mL	1.9135 mL	3.8270 mL	9.5675 mL
	20 mM	0.2870 mL	1.4351 mL	2.8703 mL	7.1757 mL
	25 mM	0.2296 mL	1.1481 mL	2.2962 mL	5.7405 mL
	30 mM	0.1914 mL	0.9568 mL	1.9135 mL	4.7838 mL
	40 mM	0.1435 mL	0.7176 mL	1.4351 mL	3.5878 mL
	50 mM	0.1148 mL	0.5741 mL	1.1481 mL	2.8703 mL
	60 mM	0.0957 mL	0.4784 mL	0.9568 mL	2.3919 mL
	80 mM	0.0718 mL	0.3588 mL	0.7176 mL	1.7939 mL
	100 mM	0.0574 mL	0.2870 mL	0.5741 mL	1.4351 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Arginine

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Publications Citing Use of MedChemExpress (MCE) L-Arginine

All NO Synthase Isoforms

All Endogenous Metabolite Isoforms

All Ligands for E3 Ligase Isoforms

Biological Activity

Chemical Information

Publications (23)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Arginine Related Classifications

Cat. No.: HY-N0455