Hoechst 33342 (Synonyms: bisBenzimide H 33342)

Name: Hoechst 33342
Brand: MedChemExpress
SKU: HY-15559
Rating: 5.0 (141 reviews)

製品番号: HY-15559 純度: 99.52%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

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CAS 番号 : 23491-52-3

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$25	在庫あり	Estimated Time of Arrival: December 31
10 mg	$38	在庫あり	Estimated Time of Arrival: December 31
25 mg	$64	在庫あり	Estimated Time of Arrival: December 31
50 mg	$95	在庫あり	Estimated Time of Arrival: December 31
100 mg	$145	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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Based on 141 publication(s) in Google Scholar

Other Forms of Hoechst 33342:

Hoechst 33342 analog 在庫あり
Hoechst 33342 trihydrochloride 在庫あり
Hoechst 33342 analog 2 お問い合わせ

顧客検証

Cell Imaging/Staining

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Mar 8;15(3):200. [Abstract]; C2C12 Cells were incubated with 1.25 μM MitoSOX in HBSS Ca/Mg for 10 min in dark at 37 °C with a 5% CO₂ atmosphere. Then, cell nuclei were stained by incubating with 1 µg/mL Hoechst in HBSS, for 10 min at 37 °C in the dark, with a 5% CO2 atmosphere.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103. [Abstract]; The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 15 December 2022, 138004.; Apoptotic cells in four groups were stained with Hoechst 33342 (for 10 min) reflected by bright blue nuclei, including hP-MSCs cultured on noncoated, L-Gel-coated (10 nM), and D-Gel coated (10 nM) plates under exposure of 500 μM H2O2 for 5 h except for the normal group, and PBS as control.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31.; OBs (red) and OCs were co-cultured, FAM-SGPA (green) were added to culture dish, incubated for 24 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 630 × ).

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31.; FAM-SGPA (green) interfered with OBs for 0.5 h, 1 h, 2 h and 4 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 400 × ).

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2022 May 10:289:115039. [Abstract]; MCF-7 cells were treated with QA root extract for 48 h and observed by fluorescence microscopy after Hoechst 33342 trihydrochloride (Hoechst, 1 μg/mL; 10 min) staining. Scale bar 100 μm.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Lipid Res. 2022 May;63(5):100207. [Abstract]; The labeling efficiency of BODIPY-loaded PLGA NPs in vitro. Fluorescence confocal images of labeled LDs in macrophages; LDs (green color, BODIPY) and nuclei (blue color, Hoechst 33342 (2 μg/mL; 2 min)); the scale bar represents 25 μm.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 4;11(10):4655-4671. [Abstract]; Hoechst 33342 (10 µg/mL) is added to the medium of cultured megakaryocytes for 2 h at 37°C. Cells are stained with the directly coupled monoclonal antibodies PE-anti-CD41 and APC-anti-Mpl for 30 min at 4°C. Ploidy is measured in the CD41+Mpl+ cell population.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Jan 22;12(1):77. [Abstract]; To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2021 Oct 4;220(10):e202011153. [Abstract]; The released centromere parts are marked by arrows (blue: Hoechst; green: MajSAT-mClover; red: H2B-mCherry). Chromatin was briefly counterstained with Hoechst 33342 (5 µg/mL) before imaging. Scale bar: 5 µm (yellow) and 2 µm (white).

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2020 Sep 1;113:289-304. [Abstract]; Hoechst 33342 staining (for 6 h) is used to distinguish apoptotic changes in H2O2-treated hP-MSCs.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091. [Abstract]; Hoechst 33342 (10 min) staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H₂O₂.

: Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2018;19(12):1193-1203. [Abstract]; PC-3 stable subline cells as indicated (empty vector or CRMP4a) were seeded on cover glass in full culture media for 24 h and then stained with iFluor555-conjugated phalloidin (red) and Hoechst33342 (blue). Cells were also subjected to immunocytofluorescent staining with anti-vinculin antibodies (green) followed by visualization with AlexaFluor®488-labeld secondary antibodies. The representative microscopic images were shown from four independent experiments.

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製品説明

IC₅₀ & Target

Dye reagent^[1]
DNA Stain^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.98 μg/mL Compound: 9	Cytotoxicity against human A549 cells assessed as reduction in cell survival after 24 hrs by resazurin assay Cytotoxicity against human A549 cells assessed as reduction in cell survival after 24 hrs by resazurin assay	[PMID: 27301676]
BEAS-2B	IC₅₀	1.95 μg/mL Compound: 9	Cytotoxicity against human BEAS2B cells assessed as reduction in cell survival after 24 hrs by resazurin assay Cytotoxicity against human BEAS2B cells assessed as reduction in cell survival after 24 hrs by resazurin assay	[PMID: 27301676]
DU-145	IC₅₀	4.25 μM Compound: Hoechst 33342	Cytotoxicity against human DU145 cells after 48 hrs by sulforhodamine B assay Cytotoxicity against human DU145 cells after 48 hrs by sulforhodamine B assay	[PMID: 25083189]
HEK293	IC₅₀	18 μM Compound: Hoechst 33342	Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting	[PMID: 22541068]
HEK293	IC₅₀	19 μM Compound: Hoechst 33342	Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting	[PMID: 22541068]
HEK293	IC₅₀	51 μM Compound: Hoechst 33342	Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting	[PMID: 22541068]
HeLa	IC₅₀	0.3 μM Compound: Hoechst 33342	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 21186067]
KB	IC₅₀	1.2 μM Compound: HOE-33342	Cytotoxicity against human epidermoid carcinoma vinblastine-selected multidrug-resistant (KBV-1) cell line Cytotoxicity against human epidermoid carcinoma vinblastine-selected multidrug-resistant (KBV-1) cell line	[PMID: 7658451]
KB 3-1	IC₅₀	0.01 μM Compound: HOE-33342	Cytotoxicity against human epidermoid carcinoma (KB3-1) cell line Cytotoxicity against human epidermoid carcinoma (KB3-1) cell line	[PMID: 7658451]
MCF7	IC₅₀	0.5 μM Compound: Hoechst 33342	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 21186067]
PC-3	IC₅₀	0.71 μM Compound: 1	Cytotoxicity towards prostatic carcinoma (PC3) cells in human Cytotoxicity towards prostatic carcinoma (PC3) cells in human	10.1016/S0960-894X(01)80831-3
RPMI 8402	IC₅₀	0.005 μM Compound: 1	Cytotoxicity towards lymphoblastoma (RPMI 8402) cells in human Cytotoxicity towards lymphoblastoma (RPMI 8402) cells in human	10.1016/S0960-894X(01)80831-3
RPMI 8402	IC₅₀	0.03 μM Compound: HOE-33342	Cytotoxicity against Human lymphoblast (RPMI 8402) cell line Cytotoxicity against Human lymphoblast (RPMI 8402) cell line	[PMID: 7658451]
RPMI 8402	IC₅₀	0.03 μM Compound: HOE-33342	Cytotoxicity against human lymphoblast cell line RPMI 8402 Cytotoxicity against human lymphoblast cell line RPMI 8402	[PMID: 8632422]
RPMI 8402	IC₅₀	0.5 μM Compound: 1	Cytotoxicity towards camptothecin resistant CPT-K5 cell line in human Cytotoxicity towards camptothecin resistant CPT-K5 cell line in human	10.1016/S0960-894X(01)80831-3
RPMI 8402	IC₅₀	0.9 μM Compound: HOE-33342	Cytotoxicity against Camptothecin-resistant (CPT -K5) cell line Cytotoxicity against Camptothecin-resistant (CPT -K5) cell line	[PMID: 7658451]
SK-MEL	IC₅₀	1.6 μM Compound: 1	Cytotoxicity towards melanoma (SK-Mel) cells in human Cytotoxicity towards melanoma (SK-Mel) cells in human	10.1016/S0960-894X(01)80831-3
U-87MG ATCC	IC₅₀	4.2 μM Compound: Hoechst 33342	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	[PMID: 21186067]

体外実験

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of Hoechst working solution
1.1 Preparation of storage solution
Prepare a 1 mg/mL storage solution of Hoechst using DMSO.
1.2 Preparation of working solution
Dilute the stored solution with pre-heated serum-free cell culture medium or PBS to achieve a final concentration of 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to collect the cells, then add PBS for two washes, each for 5 minutes. The cell density is 1×10⁶/mL
2.2 Add 1 mL of Hoechst working solution and incubate at room temperature for 3-10 minutes.
2.3 400 grams. Centrifuge for 3-4 minutes. Discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cytological staining (adherent cells)
3.1 Place the adherent cells on a sterile cover slip.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 3 to 10 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Observe using a fluorescence microscope or flow cytometer.

Notes:
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is exclusively for professional scientific research purposes only. It shall not be used for clinical diagnosis or treatment, nor for food or medicine purposes.
3. For your safety and health, please wear a laboratory coat and put on disposable gloves when operating.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

452.55

分子式

C₂₇H₂₈N₆O

Emission (Em)

452

Excitation (Ex)

356

CAS 番号

23491-52-3

Appearance

Solid

Color

Brown to breen

SMILES

CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 10 mg/mL (22.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2097 mL	11.0485 mL	22.0970 mL
5 mM	0.4419 mL	2.2097 mL	4.4194 mL
10 mM	0.2210 mL	1.1049 mL	2.2097 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.52%

Select Batch:

データシート (275 KB) SDS (393 KB)

COA (245 KB) HNMR (438 KB) RP-HPLC (95 KB) LCMS (267 KB)

取扱説明書 (2659 KB)

Dyeing Example

参考文献

[1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557. [Content Brief]

細胞実験
^[1]

Labeling Nuclear DNA with Hoechst 33342^[1] Step 1, Dilute the Hoechst stock solution 1:100 in H₂O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS⁺. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS⁺. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)^[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2097 mL	11.0485 mL	22.0970 mL	55.2425 mL
	5 mM	0.4419 mL	2.2097 mL	4.4194 mL	11.0485 mL
	10 mM	0.2210 mL	1.1049 mL	2.2097 mL	5.5243 mL
	15 mM	0.1473 mL	0.7366 mL	1.4731 mL	3.6828 mL
	20 mM	0.1105 mL	0.5524 mL	1.1049 mL	2.7621 mL

Hoechst 33342 Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hoechst 3334223491-52-3bisBenzimide H 33342 HOE 33342Hoechst33342Hoechst-33342bisBenzimide H33342bisBenzimide H-33342HOE33342HOE 33342HOE-33342AutophagyHoechstDNAcellular dyeblue fluorescenceInhibitorinhibitorinhibit

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Hoechst 33342 (Synonyms: bisBenzimide H 33342)

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Other Forms of Hoechst 33342:

Hoechst 33342 関連抗体

顧客検証

おすすめ

•関連小分子:

•関連タンパク質:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Hoechst 33342 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hoechst 33342 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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