2. MCE-Kits
  3. Molecular Biology
  4. Restriction Endonuclease
  5. Dpn I

Dpn I 

Art. -Nr.: HY-KE7011
Manual COA SDS Technical Support
Expression Purification

MCE Dpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mal I.

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  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

•    Dpn I is 100% active in 10× Buffer and 10× Color Buffer. 10× Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the Color Buffer migrates with 2500 bp DNA fragments in a 1% agarose gel and the yellow dye of the Color Buffer migrates faster than 10 bp DNA fragments in a 1% agarose gel.

 

•   Cleavage site:

 

5’…G      Am6▼T      C…3’


3’…C      T   ▲  Am6 G…5’
Speicherung

-20°C, 2 years.
Komponenten
Components HY-KE7011-50T
Dpn I 50 μL
10× Buffer 1 mL
10× Color Buffer 1 mL
Dokumentation

Manual (125 KB) SDS (251 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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