Human CD34⁺ Cells Negative Selection Kit

製品番号: HY-K0305: Manual COA SDS
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MCE Human CD34⁺ Cells Negative Selection Kit is designed for the isolation of CD34⁺ cells from human cord blood mononuclear cells (CBMC) or human peripheral blood mononuclear cells (PBMC).

容量	価格（税別)	在庫状況	数量
200 μL	$54	在庫あり	Estimated Time of Arrival: December 31
2 mL	$478	在庫あり	Estimated Time of Arrival: December 31

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: MedChemExpress Validation
CD34⁺ cells in human umbilical cord blood were sorted using the Human CD34⁺ Cells Negative Selection Kit (HY-K0305). CD34⁺ cells were enriched from human cord blood mononuclear cells (CBMCs). Cells before and after sorting were stained with FITC-conjugated anti-human CD45 (clone HI30) and PE-conjugated anti-human CD34 antibody (clone 581) for flow cytometric analysis. The results showed that the proportion of CD34⁺ cells increased from 1.0% (pre-sorting) to 12.0% after the first sort, and further to 27.5% after the second sort.

説明
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Description
& Advantages

MCE Human CD34⁺ Cells Negative Selection Kit is designed for the isolation of CD34⁺ cells from human cord blood mononuclear cells (CBMC) or human peripheral blood mononuclear cells (PBMC). Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD34⁺ cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD34⁺ cells.

Features of MCE Human CD34⁺ Cells Negative Selection Kit

• Efficiency: The enrichment of CD34⁺ cells completes in just 30 minutes;

• Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

• High Enrichment Efficiency: The process enables one-step enrichment with up to a 10× concentration, and two-step enrichment offering up to a 30× concentration.

保管条件

4°C，2 years

Do not freeze the magnetic beads

成分

Components	HY-K0305-0.2 mL (For 10⁸ cells)	HY-K0305-2 mL (For 10⁹ cells)
Biotin-Antibody Mix	20 μL	200 μL
Streptavidin Magnetic Beads	0.2 mL	1 mL × 2

ドキュメンテーション

Manual (165 KB) SDS (86 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

human CD34+ T Cell Kithuman CD34+ T Cell Sorting Magnetic BeadsCD34+ T cell Microbeads

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Human CD34+ Cells Negative Selection Kit

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最近チェックした製品:

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Human CD34⁺ Cells Negative Selection Kit