IRBP (1-20), human TFA

Name: IRBP (1-20), human TFA
Brand: MedChemExpress
SKU: HY-P1587A
Rating: 4.5 (0 reviews)

製品番号: HY-P1587A 純度: 98.12%: Data Sheet SDS COA 取扱説明書
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IRBP (1-20), human TFA contains a major epitope for the H-2^b haplotype. IRBP (1-20), human TFA induces experimental autoimmune uveoretinitis (EAU) in H-2^b mice.

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IRBP (1-20), human TFA 構造式

容量	価格（税別）	在庫状況	数量
5 mg	$480	在庫あり	Estimated Time of Arrival: December 31
10 mg	$800	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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IRBP (1-20), human 在庫あり

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製品説明

IRBP (1-20), human TFA contains a major epitope for the H-2^b haplotype. IRBP (1-20), human TFA induces experimental autoimmune uveoretinitis (EAU) in H-2^b mice^[1]^[2].

体内実験

IRBP (1-20), human TFA induces EAU^[2].
For determination of proliferative and cytokine responses, lymph node and spleen cells are collected 21 days after immunization and are stimulated in culture with the appropriate antigen. Cells of C57BL/6 mice and 129/J mice immunized with IRBP (1-20), human TFA proliferated in response to the peptide^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (H-2^b) mice, H-2b (C57BL/6, 129/J) mice^[2]
Dosage:	200, 300 μg
Administration:	I.h.; After 21 days
Result:	C57BL/6 (H-2^b) mice immunized with the human peptide (200-300 μg= 80-120 nmoles) developed EAU. 129/J mice (H-2^b) also developed disease, albeit with a lower average score than C57BL/6.

分子量

2308.62

分子式

C₁₀₃H₁₆₅F₃N₂₄O₃₀S

Appearance

Solid

Color

White to off-white

Sequence

Gly-Pro-Thr-His-Leu-Phe-Gln-Pro-Ser-Leu-Val-Leu-Asp-Met-Ala-Lys-Val-Leu-Leu-Asp

Sequence Shortening

GPTHLFQPSLVLDMAKVLLD

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Sealed storage, away from moisture
Powder	-80°C	2 years
	-20°C	1 year
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (43.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 8.33 mg/mL (3.61 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4332 mL	2.1658 mL	4.3316 mL
5 mM	0.0866 mL	0.4332 mL	0.8663 mL
10 mM	0.0433 mL	0.2166 mL	0.4332 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (1.08 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 98.12%

Select Batch:

データシート (270 KB) SDS (252 KB)

COA (241 KB) RP-HPLC (96 KB) MS (210 KB) Elemental Analysis Report (100 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Avichezer D, et al. Residues 1-20 of IRBP and whole IRBP elicit different uveitogenic and immunological responses in interferon gamma deficient mice. Exp Eye Res. 2000;71(2):111‐118. [Content Brief]

[2]. Avichezer D, Silver PB, Chan CC, Wiggert B, Caspi RR. Identification of a new epitope of human IRBP that induces autoimmune uveoretinitis in mice of the H-2b haplotype. Invest Ophthalmol Vis Sci. 2000;41(1):127‐131. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.4332 mL	2.1658 mL	4.3316 mL	10.8290 mL
DMSO	5 mM	0.0866 mL	0.4332 mL	0.8663 mL	2.1658 mL
	10 mM	0.0433 mL	0.2166 mL	0.4332 mL	1.0829 mL
	15 mM	0.0289 mL	0.1444 mL	0.2888 mL	0.7219 mL
	20 mM	0.0217 mL	0.1083 mL	0.2166 mL	0.5414 mL
	25 mM	0.0173 mL	0.0866 mL	0.1733 mL	0.4332 mL
	30 mM	0.0144 mL	0.0722 mL	0.1444 mL	0.3610 mL
	40 mM	0.0108 mL	0.0541 mL	0.1083 mL	0.2707 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.