1. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
  2. Reactive Oxygen Species
  3. J14

J14 

Cat. No.: HY-135008 Purity: 99.45%
Handling Instructions

J14 is a reversible sulfiredoxin inhibitor with an IC50 of 8.1 μM. J14 induces oxidative stress (intracellular ROS accumulation) by inhibiting sulfiredoxin, leading to cytotoxicity and cancer cell death.

For research use only. We do not sell to patients.

J14 Chemical Structure

J14 Chemical Structure

CAS No. : 1043854-13-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 97 In-stock
Estimated Time of Arrival: December 31
5 mg USD 85 In-stock
Estimated Time of Arrival: December 31
10 mg USD 125 In-stock
Estimated Time of Arrival: December 31
25 mg USD 195 In-stock
Estimated Time of Arrival: December 31
50 mg USD 295 In-stock
Estimated Time of Arrival: December 31
100 mg USD 500 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

J14 is a reversible sulfiredoxin inhibitor with an IC50 of 8.1 μM. J14 induces oxidative stress (intracellular ROS accumulation) by inhibiting sulfiredoxin, leading to cytotoxicity and cancer cell death[1].

IC50 & Target

IC50: 8.1 μM (Sulfiredoxin); ROS[1]

In Vitro

J14 (0-100 μM; 0-96 hours; A549 cells) treatment inhibits the growth of A549 cells in a concentration- and a time- dependent manner, and its half inhibitory concentration for the growth of A549 cells was 15.7 μM[1].
J14 (20 μM; 48-72 hours; A549 cells) treatment causes not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. J14 induces oxidative damage to mitochondria, resulting in caspase-mediated apoptosis[1].
J14 treatment significantly increases the accumulation of sulfinic peroxiredoxins and intracellular ROS. Excess accumulation of intracellular ROS causes oxidative damage, leading to cell death. J14 significantly induces cell death in A549 cells in a time-dependent manner, resulting in approximately 40% cell death in 96 hours[1].
J14 induces oxidative mitochondrial damage and apoptosis[1].

Cell Viability Assay[1]

Cell Line: A549 cells
Concentration: 0-100 μM
Incubation Time: 0 hour, 24 hours, 48 hours, 72 hours, 96 hours
Result: Inhibited the growth of A549 cells in a concentration- and a time- dependent manner.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 20 μM
Incubation Time: 48 hours, 72 hours
Result: Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9.
In Vivo

J14 (50 mg/kg; intraperitoneal injection; daily; for 16 days; BALB/c nude female mice) treatment significantly reduces the average tumor volume. The masses and weights of the primary tumors excised from the J14-treated mice are significantly lower compared with those of the control mice[1].

Animal Model: Six-week-old BALB/c nude female mice injected with A549 cells[1]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection; daily; for 16 days
Result: Significantly reduced the growth of human lung tumor without acute toxicity.
Molecular Weight

517.04

Formula

C₂₈H₂₅ClN₄O₂S

CAS No.

1043854-13-2

SMILES

O=C(O)C1=CC=C(CSC2=NC(C3=CC=CC=C3)=CC(N4CCN(C5=CC=CC=C5Cl)CC4)=N2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

J14J 14J-14Reactive Oxygen SpeciesSulfiredoxincytotoxicitymitochondrialdamagecancerdeathlungadenocarcinomacellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product name:
J14
Cat. No.:
HY-135008
Quantity:
MCE Japan Authorized Agent: