JNK-IN-7
Based on 8 publication(s) in Google Scholar
JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.17%
- No. CAS: 1408064-71-0
- Fòrmula: C28H27N7O2
- Peso molecular:493.56
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) JNK-IN-7
More- J Exp Clin Cancer Res. 2023 Jul 13;42(1):166. [Abstract]
- Sci Adv. 2020 May 22;6(21):eaaz8521. [Abstract]
- J Allergy Clin Immunol. 2019 Oct;144(4):1036-1049. [Abstract]
- Transl Psychiatry. 2025 Jul 11;15(1):239. [Abstract]
- Neurobiol Dis. 2025 Jan:204:106763. [Abstract]
- Transl Oncol. 2025 Aug:58:102436. [Abstract]
- ACS Infect Dis. 2026 Jun 12;12(6):2015-2024. [Abstract]
- University of California. 2019 Nov.
Actividad biológica
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JNK3 0.7 nM (IC50) |
JNK1 1.5 nM (IC50) |
JNK2 2 nM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
0.244 μM
Compound: 71
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Inhibition of JNK3-mediated c-jun phosphorylation in human A375 cells after 1 hr incubation
Inhibition of JNK3-mediated c-jun phosphorylation in human A375 cells after 1 hr incubation
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[PMID: 25415535] |
| HeLa | IC50 |
0.13 μM
Compound: 71
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Inhibition of JNK3-mediated c-jun phosphorylation in human HeLa cells after 1 hr incubation
Inhibition of JNK3-mediated c-jun phosphorylation in human HeLa cells after 1 hr incubation
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[PMID: 25415535] |
JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48 h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1408064-71-0
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Appearance Solid
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Peso molecular 493.56
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Fòrmula C28H27N7O2
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Color White to gray
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SMILES
O=C(C1=CC(NC(/C=C/CN(C)C)=O)=CC=C1)NC(C=C2)=CC=C2NC3=NC=CC(C4=CN=CC=C4)=N3
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Synonyms
JNK inhibitor
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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J Exp Clin Cancer Res
Pulmonary interleukin 1 beta/serum amyloid A3 axis promotes lung metastasis of hepatocellular carcinoma by facilitating the pre-metastatic niche formation. [Abstract]2023 Jul 13;42(1):166. PMID: 37443052 -
Sci Adv
Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy. [Abstract]2020 May 22;6(21):eaaz8521. PMID: 32494745 -
J Allergy Clin Immunol
2019 Oct;144(4):1036-1049. PMID: 31378305 -
Transl Psychiatry
Integrative analysis identifies IL-6/JUN/MMP-9 pathway destroyed blood-brain-barrier in autism mice via machine learning and bioinformatic analysis. [Abstract]2025 Jul 11;15(1):239. PMID: 40645949 -
Neurobiol Dis
Unraveling pathogenesis and potential biomarkers for autism spectrum disorder associated with HIF1A pathway based on machine learning and experiment validation. [Abstract]2025 Jan:204:106763. PMID: 39657846 -
Transl Oncol
KAT5 promotes tumor growth in androgen-independent prostate cancer by facilitating aerobic glycolysis. [Abstract]2025 Aug:58:102436. PMID: 40483992 -
ACS Infect Dis
Convergent Activation of NF-κB and STAT3 Drives Cartilage Degeneration in a Murine Model of BCG-Induced Arthritis. [Abstract]2026 Jun 12;12(6):2015-2024. PMID: 42210531 -
Solvente y solubilidad
DMSO : 33.33 mg/mL (67.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (5.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.75 mg/mL (5.57 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Intestinal epithelial cell line (HCT116) is cultured in DMEM medium. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1 μM), NF-κB inhibitor (BAY 11-7082, 1 μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50 μM) are also added into the culture medium. After 48 h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54. [Content Brief]
[2]. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0261 mL | 10.1305 mL | 20.2610 mL | 50.6524 mL |
| 5 mM | 0.4052 mL | 2.0261 mL | 4.0522 mL | 10.1305 mL | |
| 10 mM | 0.2026 mL | 1.0130 mL | 2.0261 mL | 5.0652 mL | |
| 15 mM | 0.1351 mL | 0.6754 mL | 1.3507 mL | 3.3768 mL | |
| 20 mM | 0.1013 mL | 0.5065 mL | 1.0130 mL | 2.5326 mL | |
| 25 mM | 0.0810 mL | 0.4052 mL | 0.8104 mL | 2.0261 mL | |
| 30 mM | 0.0675 mL | 0.3377 mL | 0.6754 mL | 1.6884 mL | |
| 40 mM | 0.0507 mL | 0.2533 mL | 0.5065 mL | 1.2663 mL | |
| 50 mM | 0.0405 mL | 0.2026 mL | 0.4052 mL | 1.0130 mL | |
| 60 mM | 0.0338 mL | 0.1688 mL | 0.3377 mL | 0.8442 mL |
JNK-IN-7 Related Classifications
HY-15617 Related Classifications
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