1. MAPK/ERK Pathway
  2. JNK
  3. JNK-IN-7

JNK-IN-7 (Synonyms: JNK inhibitor)

Cat. No.: HY-15617 Purity: 98.05%
Handling Instructions

JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.

For research use only. We do not sell to patients.

JNK-IN-7 Chemical Structure

JNK-IN-7 Chemical Structure

CAS No. : 1408064-71-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 377 In-stock
Estimated Time of Arrival: December 31
5 mg USD 343 In-stock
Estimated Time of Arrival: December 31
10 mg USD 443 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1195 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1906 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
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Based on 1 publication(s) in Google Scholar

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Description

JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.

IC50 & Target[1]

JNK3

0.7 nM (IC50)

JNK1

1.5 nM (IC50)

JNK2

2 nM (IC50)

In Vitro

JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48 h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].

Molecular Weight

493.56

Formula

C₂₈H₂₇N₇O₂

CAS No.

1408064-71-0

SMILES

O=C(C1=CC(NC(/C=C/CN(C)C)=O)=CC=C1)NC(C=C2)=CC=C2NC3=NC=CC(C4=CN=CC=C4)=N3

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (67.53 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0261 mL 10.1305 mL 20.2610 mL
5 mM 0.4052 mL 2.0261 mL 4.0522 mL
10 mM 0.2026 mL 1.0130 mL 2.0261 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.75 mg/mL (5.57 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.75 mg/mL (5.57 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

Intestinal epithelial cell line (HCT116) is cultured in DMEM medium. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1 μM), NF-κB inhibitor (BAY 11-7082, 1 μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50 μM) are also added into the culture medium. After 48 h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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JNK-IN-7
Cat. No.:
HY-15617
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