Leucocrystal violet-d₆ 

Leucocrystal violet-d₆ is the deuterium labeled Leucocrystal violet (HY-D0233). Leucocrystal violet is a cationic triarylmethane dye. Leucocrystal violet generates a colored purple product in the presence of hydrogen peroxide and horseradish peroxidase, or when oxidized by periodate under the catalysis of horseradish peroxidase. Leucocrystal violet serves as a chromogenic reagent for the determination of submicrogram-level hydrogen peroxide, with no interference from tryptophan, persulfate or chromate. Leucocrystal violet reacts with blood to produce a purple/violet color in the presence of hydrogen peroxide; it forms a very weak fluorophore when acting with whole blood in an unbuffered solution, with a maximum absorption wavelength of 630 nm and a maximum fluorescence emission wavelength of 665 nm upon excitation at 630 nm. Leucocrystal violet is widely used for staining blood residues on porous and non-porous materials.

1. This compound can be used as a tracer.
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

379.57

C₂₅H₂₅D₆N₃

1173023-92-1

603-48-5

[2H]C([2H])([2H])N(C([2H])([2H])[2H])C(C=C1)=CC=C1C(C2=CC=C(C=C2)N(C)C)C3=CC=C(C=C3)N(C)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216.  [Content Brief]

  [2]. Wu J, et al. Biodegradation of leuco derivatives of triphenylmethane dyes by Sphingomonas sp. CM9. Biodegradation. 2011 Sep;22(5):897-904.  [Content Brief]

  [3]. Tiwari KK, et al. A simple and sensitive analytical method for the determination of antimony in environmental and biological samples. Anal Sci. 2006 Feb22(2):259-62.  [Content Brief]