2. Academic Validation
  3. Newcastle disease virus-like particles induce DC maturation through TLR4/NF-κB pathway and facilitate DC migration by CCR7-CCL19/CCL21 axis

Newcastle disease virus-like particles induce DC maturation through TLR4/NF-κB pathway and facilitate DC migration by CCR7-CCL19/CCL21 axis

  • Vet Microbiol. 2017 May;203:158-166. doi: 10.1016/j.vetmic.2017.03.002.
Jing Qian 1,  Xiaohong Xu 1,  Jiaxin Ding 1,  Renfu Yin 1,  Yixue Sun 2,  Cong Xue 1,  Jianzhong Wang 2,  Chan Ding 3,  Shengqing Yu 3,  Xiufan Liu 4,  Shunlin Hu 4,  Chunfeng Wang 2,  Yanlong Cong 5,  Zhuang Ding 6
Affiliations

Affiliations

  • 1 Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
  • 2 Engineering Research Center of Jilin Province for Animals Probiotics, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
  • 3 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
  • 4 Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
  • 5 Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China. Electronic address: [email protected].
  • 6 Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China. Electronic address: [email protected].
PMID: 28619138 DOI: 10.1016/j.vetmic.2017.03.002
Abstract

Newcastle disease virus-like particles (NDV VLPs) are a potential candidate vaccine, as shown by eliciting specific immune response against NDV in mice and chickens. Activation of dendritic cells (DCs) is critical to initiate immune response. However, the mechanism of how NDV VLPs induce DC maturation and migration remains elusive. In this study, we found that NDV VLPs are efficient in DC activation by up-regulating surface MHC II and costimulatory molecules, and proinflammatory cytokines through the TLR4/NF-κB pathway. Furthermore, NDV VLPs elevated CCR7 expression on DCs, resulting in DC migration towards CCL19/CCL21 both in vitro and ex vivo. As a consequence of DC maturation and migration, CD4+ T cells were also activated in vivo, demonstrating increased intracellular IFN-γ and IL-4 levels. Together, these results present new insights for NDV VLPs induced DC maturation and migration, providing a better understanding of VLP-triggered innate immune responses.

Keywords

Dendritic cells; Innate immunity; Maturation; Migration; Newcastle disease virus-like particles.

Figures
Products