2. Academic Validation
  3. Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35-55 in the Mouse

Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35-55 in the Mouse

  • Methods Mol Biol. 2018;1791:227-232. doi: 10.1007/978-1-4939-7862-5_17.
Mercedes Giralt 1 2,  Amalia Molinero 1 2,  Juan Hidalgo 3 4
Affiliations

Affiliations

  • 1 Institute of Neurosciences, Universitat Autònoma de Barcelona, Barcelona, Spain.
  • 2 Department of Cellular Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
  • 3 Institute of Neurosciences, Universitat Autònoma de Barcelona, Barcelona, Spain. [email protected].
  • 4 Department of Cellular Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. [email protected].
PMID: 30006713 DOI: 10.1007/978-1-4939-7862-5_17
Abstract

Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the Materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) + complete Freund's adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG35-55.

Keywords

EAE; Experimental autoimmune encephalomyelitis; MOG35–55; Multiple sclerosis; Myelin oligodendrocyte glycoprotein.

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