MOG (35-55) TFA
Based on 9 publication(s) in Google Scholar
MOG (35-55) (MOG (35-55)) TFA is a minor component of CNS myelin. MOG (35-55) (TFA) has encephalitogenic activity and induces T cell proliferative. MOG (35-55) (TFA) induces Th1 cytokine response as well as relatively high levels of IgG antibodies. MOG (35-55) (TFA) produces a relapsing-remitting neurological disease with extensive plaque-like demyelination. MOG (35-55) (MOG (35-55)) TFA can be used for experimental autoimmune encephalomyelitis (EAE) modeling.
For research use only. We do not sell to patients.
- Purity : 99.68%
- Formula: C120H178F3N35O31S
- Molecular Weight:2695.97
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MOG (35-55) TFA
More- Adv Sci (Weinh). 2025 Apr;12(14):e2409086. [Abstract]
- Cell Death Dis. 2022 Sep 2;13(9):759. [Abstract]
- EMBO Mol Med. 2025 Sep 29. [Abstract]
- Acta Physiol. 2023 Jun;238(2):e13974. [Abstract]
- Int Immunopharmacol. 2022 Apr:105:108566. [Abstract]
- Front Biosci (Landmark Ed). 2025 May 23;30(5):37838. [Abstract]
- Mol Biol Rep. 2025 Nov 15;53(1):92. [Abstract]
- Cornell University. 2025.
- Research Square Print. September 28th, 2022.
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Others
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Histological Imaging/Staining
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Others
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Cell Proliferation/Viability Assay
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ELISA
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Biological Activity
Description
In Vitro
MOG (35-55) (MOG (35-55); 0-50 μg/mL; 72 h; lymph nodes cells) TFA induces T cell proliferative and secretes Th1 cytokines including IFN-γ, TNF-α, IL-10, IL-4 and IL-5. MOG (35-55) (TFA) increases the level of IgG[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
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Animal Model:HLA-DR2 (DRB1*1501) mice[1]
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Dosage:200 μg (0.2 mL)
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Administration:Intraperitoneal injection; once, for 38 days
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Result:Resulted in paralysis of both hind and forelimbs.
Induced Disease Models
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 is one of the targets of self-reactive T cell responses, leading to acute and later chronic autoimmune processes. MOG 35-55 is capable of inducing experimental autoimmune encephalomyelitis (EAE) in B6 mice[1].
Administration: On day 0: 3 mg/mL peptide plus CFA (4 mg/mL) • Mix thoroughly 1: 1 peptide and CFA solutions • s.c. • 100 μL for each hind flank; • On day 0 and day 2: 2.5 μg/mL of Bordetella pertussis toxin • i.p. • 200 μL
2. Symptoms appear approximately 10-15 days after immunization.
3. When mice have clinical symptoms of EAE, hydrated food is placed on the cage floor to facilitate access to these mice.
4. Record parameters every other day at the beginning, and daily from 7th postimmunization day. Body weight decreases usually precedes EAE clinical signs and is a very objective and valuable measure.
prototypical histopathological changes: Luxol fast blue staining of spinal cord for myelin evaluation shows infiltrates and demyelination in EAE animals.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 2695.97
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Formula C120H178F3N35O31S
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Color White to off-white
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Synonyms
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA
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Sequence
Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys
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Sequence Shortening
MEVGWYRSPFSRVVHLYRNGK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (9)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling. [Abstract]2025 Apr;12(14):e2409086. PMID: 39804990 -
Cell Death Dis
Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling. [Abstract]2022 Sep 2;13(9):759. PMID: 36055989 -
EMBO Mol Med
Selective inhibition of mitochondrial Kv1.3 prevents and alleviates multiple sclerosis in vivo. [Abstract]2025 Sep 29. PMID: 41023199
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Sep 29. [Abstract]
Scheme of the EAE Adoptive Transfer Model: Mice underwent immunization via subcutaneous injection of MOG35–55 in complete Freund’s adjuvant. Ten days post-immunization, spleens were harvested, and single-cell suspensions were prepared. These splenocytes were treated for 3 days with IL-12, IL-23, and anti-IFN-γ. Subsequently, the splenocytes were subjected to a 3-day treatment with 1 μM PAPTP, or left untreated. Antigen-specificity of the cells was confirmed by additional proliferation assays of isolated donor lymphocytes (see Methods). Lymphocytes were isolated, and the induction of apoptosis was assessed using flow cytometry. Additionally, these lymphocytes were transferred into wild-type recipient animals, and the animals’ clinical scores were monitored daily for 10 days. Following the observation period, the animals were euthanized for further analysis.
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Sep 29. [Abstract]
Representative image (of 5 sections) of brains (upper part of the pons region) stained with PE-anti-CD45, from wild-type mice intraperitoneally injected with either untreated or PAPTP 1 μM treated, MOG35–55 activated lymphocytes.3J
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Sep 29. [Abstract]
Treatment scheme for wild-type mice immunized via subcutaneous injection of MOG35–55: upon the manifestation of initial symptoms, typically around day 10 post-immunization, treatment commenced. The treatment regimen was administered every 48 h, totaling 11 injections. Mice were euthanized the day following the final injection for subsequent analysis of peripheral blood, brains, and spinal cords.
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Acta Physiol
SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice. [Abstract]2023 Jun;238(2):e13974. PMID: 37186158 -
Int Immunopharmacol
High-density lipoprotein reduces microglia activation and protects against experimental autoimmune encephalomyelitis in mice. [Abstract]2022 Apr:105:108566. PMID: 35101849 -
Front Biosci (Landmark Ed)
HMGB1 Mediates Microglia-Astrocyte/Neuron Crosstalk and Pyroptosis by the TLR4/NF-κB Pathway in Multiple Sclerosis. [Abstract]2025 May 23;30(5):37838. PMID: 40464519
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 May 23;30(5):37838. [Abstract]
The BV-2 cells were cultured in designated concentrations of MOG35-55 (0-25μg/mL, 24 h). MTT analysis of BV-2 cell viability.
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 May 23;30(5):37838. [Abstract]
The BV-2 cells were cultured in designated concentrations of MOG35-55 (0-25μg/mL, 24 h). ELISA analysis of the release of HMGB1.
MOG (35-55) TFA purchased from MedChemExpress. Usage Cited in: Front Biosci (Landmark Ed). 2025 May 23;30(5):37838. [Abstract]
The BV-2 cells were cultured in designated concentrations of MOG35-55 (0-25μg/mL). Cytometry analysis for CD11b+ and CD68+ cells.
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Mol Biol Rep
Th2 cell-derived exosomes alleviate central nervous system inflammation and demyelination in experimental autoimmune encephalomyelitis by regulating inflammasomes to inhibit microglia activation. [Abstract]2025 Nov 15;53(1):92. PMID: 41240231 -
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Solvent & Solubility
In Vitro:
H2O : ≥ 50 mg/mL (18.55 mM)
DMSO : 2 mg/mL (0.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (37.09 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Rich C, et, al. Myelin oligodendrocyte glycoprotein-35-55 peptide induces severe chronic experimental autoimmune encephalomyelitis in HLA-DR2-transgenic mice. Eur J Immunol. 2004 May;34(5):1251-61. [Content Brief]
[2]. Slavin A, et, al. Induction of a multiple sclerosis-like disease in mice with an immunodominant epitope of myelin oligodendrocyte glycoprotein. Autoimmunity. 1998;28(2):109-20. [Content Brief]
[3]. Giralt M, et, al. Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35-55 in the Mouse. Methods Mol Biol. 2018;1791:227-232. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3709 mL | 1.8546 mL | 3.7092 mL | 9.2731 mL |
| 5 mM | 0.0742 mL | 0.3709 mL | 0.7418 mL | 1.8546 mL | |
| 10 mM | 0.0371 mL | 0.1855 mL | 0.3709 mL | 0.9273 mL | |
| 15 mM | 0.0247 mL | 0.1236 mL | 0.2473 mL | 0.6182 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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